摘要
目的:提取阿魏菇中的多糖并测定其含量,初步研究阿魏菇多糖的急性毒性及抗炎作用。方法:以水提醇沉法提取阿魏菇多糖,应用苯酚-硫酸比色法测多糖含量,采用最大给药量法测定阿魏菇多糖对小鼠的最大耐受量,抗炎实验采用二甲苯致鼠耳肿胀法。结果:阿魏菇多糖提取率分别为11.23%,总多糖含量为44.57%;急性毒性试验测得阿魏菇多糖的小鼠灌胃最大耐受量分别为212.39g生药/kg,相当于人日用量的2137倍;抗炎试验表明,阿魏菇多糖高、低剂组均能显著抑制二甲苯所致小鼠耳肿胀。结论:阿魏菇多糖提取工艺简便,安全无毒,并且具有一定的抗炎作用。
Objective： To extract and study the anti-inflammation effect and acute toxicity of the polysaccharide from Pleurotus fer-ulae Lanzi. Methods：The polysaccharide of Pleurotus ferulae Lanzi （PfP） was extracted by water, precipitated by ethanol and the content of the polysaccharide was determined by the phenol-sulfuric acid colorimetric method. The maximum administration dosage （MTD） of PfP was determined by ig intragastric administration with the maximum concentration and the maximum amounts in mice, and anti- inflammatory model of mouse ear edema induced by xylene. Results：The yield of PfP was 11.23 % and the contents of total polysaccharide was 44.57%. The MTD test showed that the MTD of PfP was 213.51g/kg, equal to 2137 times of the normal hu-man（50kg）. PfP could inhibit the mice ear edema induced by xylene. Conclusion： The extraction process of PfP is simple. PfP is safe in clinic use and has some certain effect on anti-inflammation.
出处
《亚太传统医药》
2013年第2期7-8,共2页
Asia-Pacific Traditional Medicine
关键词
阿魏侧耳
多糖
含量测定
急性毒性
抗炎
Pleurotus ferulae Lanzi
Polysaceharide
Content Determination
Acute Toxicity
Anti-inflammation
