摘要
目的 建立用单克隆抗体技术检测细胞内IL 1ra的方法。方法 制备异硫氰酸荧光素 (FITC)标记的抗人IL 1ra单克隆抗体 ,使其进入U937细胞内与icIL 1ra结合 ,然后用流式细胞仪 (FACS)检测荧光强度 ,并比较U937细胞在刺激前后荧光强度的变化情况是否与icIL 1ra表达的变化情况相一致。结果 在一定剂量范围内 ,经PMA分化和LPS刺激的U937细胞的icIL 1ra表达量增加 ,其检测荧光强度相应增加。结论 用单克隆抗体检测细胞内icIL 1ra是一种快速、准确的方法 ,本实验方法能够满足实际研究工作中对icIL
Objective To establish a method to measure the intracellu lar IL 1ra (icIL 1ra) using monoclonal antibody technique. Meth ods Anti human IL 1ra monoclonal antibody (McAb) was labeled with fluorescei n isothiocyanate (FITC), and Fluorescence Activated Cell Sorter (FACS) measured fluorescence intensity after FITC labeled anti human IL 1ra McAb bound with U 937 cells. The changes of the fluorescence intensity were compared with the chan ges of the icIL 1ra level in the U937 cells that were stimulated and not. Results The fluorescence intensity rose along with the increase of i cIL 1ra level in PMA differentiated and LPS stimulated U937 cells within the range. Conclusion The method of measuring icIL 1ra using McAb technique is rapi d and precision. It can meet the demands of quantifying icIL 1ra of the researc h.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第3期230-233,共4页
Immunological Journal
