摘要
以杜氏利什曼前鞭毛体为靶抗原的L12G9单克隆抗体(McAb),检测人工感染杜氏利什曼原虫的白蛉。当空腹雌蛉吸取病鼠血液后,分别饲养4、6、8和10d后解剖。吸血后4d,前鞭毛体较少。阳性率仅为15.9％;而10d,其感染程度较重,可获100％的阳性检出率。其阳性率与白蛉的感染程度呈正相关。另外又证明应用单克隆抗体检测时,原虫数不能低于11×10~7/ml,宜选择胃血完全消化后的白蛉,方可得到良好的效果。如捕获的白蛉,胃内前鞭毛体较少,则可经NNN基培养后,再进行检测,亦可获得同样效果。
It has been reported by the authors that monoclonal antibody L12G9 produced from target antigens of L. donovani promastigotes, was very useful for detecting promastigotes from artificially infected sandflies. In the present study, detection of promastigotes from artificially infected sandflies by McAb showed that the positive rate correlated with the infection duration of sandflies. 4 days after feeding on infected Chinese hamsters, the sandflies were lightly infected with L. donovani promastigotes with a positive rate of 15.9%, but 10 days later, the sandflies were heavily infected, the positive rate being 100%:Observation has been made on the relationship between the number of promastigotes and mouse blood dilution. The results showed that satisfactory results could be obtained by using monoclonal antibodies in the detection of L. donovani, the number of promas tigotes should be over 1 × 107/ml, and the blood meals in sandflies completely digested.If very few promastigotes were present in naturally infected sandflies before identification by monoclonal antibody, the parasites must be grown in NNN medium. Positive result could then be obtained.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1990年第4期274-276,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家"七五"科技攻关专题资助
关键词
单克隆抗体
前鞭毛体
白蛉
Monoclonal antibody, promastigote, sandfly, Leishmania donovant.
