摘要
采用点印迹法与Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ技术对ｒＩＬ－１ｒａＭｃＡｂｓ进行了鉴定。结果表明：抗ｒＩＬ－１ｒａＭｃＡｂ２Ｅ４在点印迹中识别信号为阳性（＋＋），经转移电泳后可在ＮＣ膜上特异性识别ｒＩＬ－１ｒａ（２０ｋｄ）；ＰｃＡｂ对照识别结果进一步表明Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ技术的高度敏感性及ＭｃＡｂ２Ｅ４的高度特异性。此株单抗的鉴定成功将有助于进一步认识ＩＬ－１的生物学活性，特别是在炎症疾病中的病理作用。
Using dotimmunoblot and Western blotting techniques, we have identified the specificity of antirIL1ra McAbs. The results showed that the antirIL1ra McAb 2E4 specifically reacted with rIL1ra, from which the recognizing signal of dotimmunoblot was significantly positive (++) and a single rIL1ra protein band (20kd) was clearly recognized by Western blotting.The control results of PcAb recognization have further proved the high sensitivity of Western blotting and high specificity of rIL1ra McAb 2E4. The successful identification of rIL1ra McAb 2E4 will be helpful to further understand the biologic effects, especially the proinflammatory properties, to study the change of rIL1ra level during inflammation, and to instruct the clinical use of rIL1ra.
出处
《华西医科大学学报》
CSCD
1997年第4期395-397,共3页
Journal of West China University of Medical Sciences
