摘要
结合作者的工作实际 ,着重介绍了有效提高PCR产物的几种方法 :如怎样高效快速地设计PCR引物 ,怎样尽快确定PCR反应的最适退火温度 ,如何提高GC富集区的扩增效率等 ,为分子生物学工作者提供一些可以借鉴的方法及经验。
Combining the author's working experiences, we introduce here several methodologies on improving the PCR products, such as the disciplines which we should abide by when we design PCR primers; how to determine the appropriate annealing temperature of the PCR reaction; how to improve the amplifying efficiency of the GC rich region. We hope to provide some methodologies for those who do research in microbiology.
出处
《生物技术通报》
CAS
CSCD
2003年第2期33-35,共3页
Biotechnology Bulletin
