摘要
目的探讨CYP2C19基因型与奥美拉唑治疗消化道溃疡的效果之间是否存在明确的相关性,以期为临床用药提供指导。方法收集北京郊区二级医院胃十二指肠溃疡病史患者200例,使用基因组DNA快速提取试剂盒,采用RealtimePCR方法,分析CYP450家族代谢酶的基因多态性;使用ABI7500fas型荧光定量PCR仪,对P450的6种基因型进行分析判读,用直接计数法计算其基因型频率及等位基因突变频率,运用Hardy-Weinberg平衡定律对基因型分布吻合度进行检验,并对基因型间有无性别差异,等位基因突变频率有无差异进行χ2检验。结果服用奥美拉唑治疗胃十二指肠溃疡的效果与其基因型相吻合,慢代谢型患者使用奥美拉唑效果及pH值提高时间明显优于快代谢组;慢代谢组使用奥美拉唑效果更好。结论北京郊区汉族人群P450家族代谢酶的三种基因类型间无性别差异,与欧洲白种人基因突变频率存在明显差异。其中,CYP3A4*18未检出纯合型突变,CYP450 2C19的基因型是影响奥美拉唑效果的重要因素。
Objective To study CYP2C19＊2 and ＊3 genotype with peptic ulcer patients using omeprazole in order to confirm therapeutic effect on CYP2C19 genotype with omeprazole whether there is a clear correlation and to guide clinical drug use. Methods 200 patients with gastroduodenal ulcer disease from Beijing outskirts secondary hospital were recruited, genomic DNA quick extraction kit and realtime-polymerase chain reaction （PCR） were used,the family of CYP450 gene polymorphism of metabolic enzymes was analyzed,ABI7500fas type fluorescent quantitative PCR was employed.Six genotypes of the P450 were analyzed , direct counting method was used to calculate the mutation genotype frequency and allele frequency. Hardy Weinberg of genotype distribution law was used to examine alignment and the presence of gender differences between genotypes and alleles mutation frequency difference by chi-square test. Results The effect of omeprazole treating gastroduodenal ulcer coincides with its genotype, the effect of omeprazole in patients with slow metabolism and pH increase time were remarkably better than patients with quick metabolism. Conclusions P450 family metabolic enzymes of the Han people in suburb Beijing have gene polymorphisms,but no gender differences between three types of gene.There exists obvious difference in the white European gene mutation frequency, and in CYP3A4＊18 the homozygous mutations are not detected , 3A4＊18 gene is not the main factor of 3A4 enzyme activity change. Omeprazole treatment effect is consistent with its genotype, CYP450 2C19 genotype is an important factor affecting the effects of omeprazole.
出处
《武警医学》
CAS
2014年第2期142-147,共6页
Medical Journal of the Chinese People's Armed Police Force
基金
武警总医院苗圃基金(WZ2011046)
