摘要
采用聚合酶链反应(PCR)的方法扩增了A rthrobacter g lobiform is中酯酶编码基因,与载体质粒连接后在大肠杆菌BL 21(DE 3)中进行表达。以包涵体形式表达的蛋白在8 m o l/L尿素作用下溶解,经过透析复性后获得了具有活性的粗蛋白。产物活性实验表明,酯酶表现出较高的催化活性,为菊酯作为杀虫剂的应用奠定了基础。
A novel esterase which stereoselectively hydrolyzes the （＋）-trans stereoisomer of ethyl chrysanthemate to produce （＋）-trans-chrysanthemic acid was discussed. The gene coding for the esterase was amplified from Arthrobacter globiformis by polymerase chain reaction （PCR）. The gene was inserted into a vector and expressed in Escherichia coli BL21 （DE3）. The protein expressed in body was dissolve in 8 mol/L urea before dialysis. The purified crude enzyme show high catalysis activity. It may suggest the possibility that （＋）-trans-chrysanthemate is identified as the insecticide.
出处
《华东理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2006年第8期930-932,979,共4页
Journal of East China University of Science and Technology
关键词
酯酶基因
克隆
表达
活性测定
esterase gene
clone
expression
esterase activity
