摘要
目的:探讨和尝试一种较为迅速确定最佳退火温度的方法来扩增目的基因。方法:采用梯度PCR对醛固酮合酶基因(CYP11B2)进行扩增,通过凝胶呈像技术判断CYP11B2基因最佳退火温度。结果:与普通PCR相比,梯度PCR能够迅速地确定CYP11B2基因的最佳退火温度。结论:梯度PCR简单、直观、快速,适用于各种核酸分子的扩增,具有高效率、高通量的优点,有着广阔的应用前景。
Objective：To explore a method for quicker identification of the optimal annealing temperature in PCR. Methods ：Gradient PCR was employed in analysis of aldosterone synthase （CYPllB2）gene polymorphisms ,and gel Imaging Technology was used to determine the optimal annealing temperature. Result：Compared with normal PCR,Gradient PCR could identify the the optimal annealing temperature of aldosterone synthase gene. Conclusions：Gradient PCR is simple,efficient and rapid,with a broad application prospects, which suitable for PCR of all kinds of genes.
出处
《中国误诊学杂志》
CAS
2008年第11期2527-2529,共3页
Chinese Journal of Misdiagnostics
基金
东莞市科技基金资助项目(编号:ZC051209
ZC060401)
关键词
聚合酶链反应/方法
基因扩增
Polymerase Chain Reaction/methods
Gene Amplification
