摘要
本文报道了用大麦、油菜总DNA和玉米花粉匀浆处理授粉前后的小麦柱头导致后代性状变异的试验结果.用大麦DNA处理的后代,在株高、成熟期、穗密度、粒色、胚乳质地等性状上出现较大变异,油菜DNA处理的后代变异较小,玉米花粉匀浆处理的后代没有变异发生.发生的变异有性状变优趋势.此外还讨论了转化试验中处理方法与结果、某些性状出现高频率变异、性状变化、外源DNA转化技术应用于小麦育种等问题.
The present paper deals with a report on treating stigmas before or after pollination with exogenous DNA extracted from barley (H. vulgare )and rape (B.napus) and with pollen paste of maize to induce character variation in wheat . The results showed that the progenies form the seeds treated with DNA of barley appeared considerable variation in the characters of mature period,ear density, grain colour,endosperm quality ;and those with DNA of rape,the variation was not so obvious ;and those with pollen paste of maize,no variation engendered. The results also indicated that wheat gamete or zygote can absorb suitable exogenous genetic materal and the character change appeared.Problems concerning methods and results in transformation, some character variation at high friquency,variation for the better,applying the technique to wheat breeding improvement etc. have also been discussed in this paper .
出处
《河南职业技术师范学院学报》
1992年第3期34-39,共6页
Journal of Henan Vocation-Technical Teachers College
基金
河南省教委资助的研究项目
关键词
小麦
外源DNA
性状变异
wheat,exogenous DNA,embryo,transformation,gene enginerring
