摘要
[目的]采用改良的SDS法提取红掌基因组DNA。[方法]以4种不同花色的红掌品种为材料，分别采用改良SDS法和CTAB法提取红掌基因组DNA，用紫外分光光度计测定英DNA在A260nm和A280nm下的吸光值，根据A260nm/A280nm的比值检测DNA的纯度和浓度。[结果]用改良SDS方法提取的DNA透明且无杂质，A260nm/A280nm比值在1．6—2．0，DNA纯度较高，可满足红掌分子生物学试验的要求；用CTAB法提取的DNA呈褐色或淡黄色，A260nm/A280nm，比值小于1．6，DNA纯度较低并且浓度低，舍有大量蛋白，此方法不适于提取红掌基因组DNA。[结论J采用改良SDS法可以提取到高质量的红掌基因组DNA，所提取的DNA的纯度和浓度可以满足红掌分子生物学研究的要求。
[ Objective ] Genomic DNA of Anthurium andraeanum was extracted with modified SDS method. [ Method ] With 4 different color varieties of A, andraeanum as materials, genomie DNA of A. andraeanum was extracted with modified SDS and CTAB method. The absorbance value was measured by ultraviolet spectrophotometer at A260nm and AA280nm and the ratio of A260nm/A280nm was used to determine the DNA purity and the mass concentration. [ Result ] Genomic DNA extracted with modified SDS method was transparent and non - impurities and the ratio of A260nm/A280nm was between 1.6 - 2, 0. The DNA purity was so higher as to meet the requirements of molecular biology research. Genomic DNA extracted with CTAB method was brown or light yellow and the ratio of A260nm/A280nm was less than 1. 6, The DNA was lower in the purity as well as the conch, and had a large number of proteins. CTAB was not suitable for extracting genomic DNA from A. andraeanum. [ Conclusion] Genomic DNA of A. andraeanum with high quality could be extracted by modified SDS method and its purity and conch, could meet the requirements of molecular biology research on A. andraeanum.
出处
《安徽农业科学》
CAS
北大核心
2009年第19期8879-8880,共2页
Journal of Anhui Agricultural Sciences
基金
青岛农业大学校高层次人才启动基金(630745)
关键词
红掌
基因组DNA
DNA提取
Anthurium andraeanum
Genomic DNA
DNA extraction
