摘要
采用花粉管通道技术将抗赤星病烤烟ＣＶ８７的总ＤＮＡ导入感病烤烟ＮＣ８９，对其Ｄ_１代植株进行了田间生长性状、抗病性和烟叶品质的检测。结果显示，柱头滴加（ＤＺ）、切柱头滴加（ＱＤ）和子房注射（ＺＺ）等３种导入方法得到的Ｄ_１代植株在株高、株型、叶型、生育期等方面发生了较大的变异，变异率分别是１９．１０％，２０％和１８．２０％。３类植侏中分别有９．１０％，１０％和６．４０％的个体获得了对赤星病的抗性。在这些植株中，筛选出ＤＺ９８１１和ＱＤ９８１２在抗病性和烟叶品质上为优良植株。
The total DNA of fluecured tobacco, CV87, with resistance to alternaria alternata, was introduced into susceptible fluecured tobacco, NC89, through pollen tube pathway. Their first generation (D_1) was analyzed in field growth traits, resistance, and quality of tobacco leaf. By the three introducing methods, ovule injecting (ZZ), stigma dropping (DZ), and cutting off stigma dropping (QD), the higher ratios of variation of plant height, plant type, leaf shape, and growth period were obtained in the D_1 generation individuals, which were 19.10%, 18.20%, and 20%, respectively. Among the plants of ZZ. QD, and DZ, there were 9.10%, 10%, and 6. 40% individuals which obtained resistance to Alternaria altrnata. DZ9811 and QD9812 are the most excellent plants among these plants with variations.
出处
《东北农业大学学报》
CAS
CSCD
2000年第2期173-179,共7页
Journal of Northeast Agricultural University
关键词
烤烟
外源DNA导入
赤星病
性状变异
抗病育种
Fluecured Tobacco
Introduction of Exogenous DNA
Resistance to Alternar- ia alternata
Variation in Character
