摘要
目的 :探讨实验性肝硬化细胞生长激素受体的表达变化。方法 :采用硫代乙酰胺腹腔注射复制大鼠肝硬化模型 ,采用放射配体法、RT -PCR、图像分析检测不同阶段肝硬化肝组织和肝硬化肝细胞中生长激素受体及其mRNA的表达水平 ,并分别与正常肝组织和正常肝细胞比较。结果 :大鼠肝硬化肝组织表达生长激素受体 ,其表达数量明显少于正常肝组织 ,并且随着肝硬化的进展、肝组织胶原纤维相对含量的增加而进一步减少 ;大鼠肝硬化肝细胞生长激素受体的位点数量明显少于正常肝细胞 ,大鼠肝硬化肝组织生长激素受体mRNA的表达量也明显少于正常肝组织。结论 :大鼠肝硬化肝细胞表达生长激素受体 ,其表达水平降低 ;肝硬化越严重 ,该表达减少越明显。大鼠肝硬化肝组织生长激素受体表达下调的机制可能是肝硬化肝组织生长激素受体mRNA的表达明显减少。
AIM: To study the expression and change of growth hormone receptors in experimental cirrhotic liver in rats. METHODS: Thioacetamide-induced liver cirrhosis was developed in Sprague-Dawley rats. The expression of growth hormone receptor and its mRNA was investigated by radio-ligand binding assay, revere transcript-polymerase chain reaction (RT-PCR) and digital image analysis in liver tissue or hepatocytes taken from normal control or cirrhotic rats with different stages of cirrhosis. RESULTS: Growth hormone-specific singular binding sties, namely growth hormone receptors, were detected in liver tissue or hepatocytes from rat samples with cirrhosis. The binding capacity of growth hormone receptors in rat cirrhotic live tissue was significant lower than that in normal control, and it decreased gradually along with the accumulation of collagen in the process of formation and development of liver cirrhosis. The binding sites of growth hormone receptors in rat cirrhotic liver cells were significantly lower than that in normal control. The expression of growth hormone receptor mRNA in rat cirrhotic liver tissue was also significant lower than that in normal control. CONCLUSIONS: The growth hormone receptor was expressed in cirrhotic livers at a reduced level in isolated hepatocytes or live tissue of cirrhotic rats, and lesser expression of growth hormone receptors was found in later stage of cirrhosis. The suppressed expression of growth hormone receptor was partly due to the reduced expression of its mRNA in cirrhotic liver tissue.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第4期490-493,共4页
Chinese Journal of Pathophysiology
基金
广东省自然科学基金资助项目 (No .9842 13)
中山医科大学 2 11工程重点学科建设基金资助项目 (No .F0 0 0 0 990 75 )
