摘要
目的探讨重组生长激素对肝硬化肝细胞表达生长激素受体的调控作用。方法检测不同浓度的重组生长激素 (0、13 3、133 3、1333ng/ml)干预后大鼠肝硬化肝细胞生长激素受体的数量变化。结果培养后肝硬化肝细胞生长激素受体的数量 (10 4 /cell)分别为 0 73± 0 13、1 14± 0 17、1 2 3± 0 2 1、0 6 8± 0 10 (P <0 0 5 )。
ObjectiveTo study growth hormone activated regulation of growth hormone receptors (GHR) in cirrhotic hepatocytes. MethodsPrimary cultures of hepatocytes from cirrhotic rat were exposed to recombinant human growth hormone (rhGH) at different concentrations of 0, 13.3, 133.3,and 1?333?ng/ml, respectively. Thereafter the binding sites of hepatocytes were detected with radio-ligand binding assay. ResultsAfter culture with different rhGH concentrations, the binding sites (10 4/cell) of receptors on cirrhotic liver cells were respectively 0.73±0.13, 1.14±0.17, 1.23±0.21, and 0.68±0.10(P<0.05). ConclusionsA positive regulation of GHR was observed by stimulation with rhGH.
出处
《中华普通外科杂志》
CSCD
北大核心
2002年第2期93-95,共3页
Chinese Journal of General Surgery
基金
广东省自然科学基金 (博士启动项目 9842 13)
关键词
肝硬化
生长激素
生长激素受体
Liver cirrhosis,experimental
Somatotropin
Receptors, somatotropin
