摘要
目的 了解胰岛细胞抗体 (ICA)和谷氨酸脱羧酶抗体 (GADA)阳性率与患儿胰岛功能的关系 ,明确ICA和GADA检测对 1型糖尿病的临床意义。方法 儿童 1型糖尿病 31例均采用ELISA法测定GADA ,间接免疫荧光法测定ICA ,化学发光法测定血清C肽、胰岛素 (DPC -IMMULITE机器 )。结果 ICA和GADA的阳性率分别为 4 5 .2 %和 38.7% ,儿童 1型糖尿病患者ICA阳性率高于GADA。抗体阳性患儿空腹和餐后C肽(0 .99± 0 .36ng/ml和 2 .10± 0 .99ng/ml)及餐后胰岛素 (11.5 3± 7.11μIU/ml)明显低于抗体阴性患儿空腹C肽 (2 .19± 1.33)ng/ml,餐后C肽 (6 .18± 3.91)ng/ml和餐后胰岛素 (18.6 0± 10 .5 9) μIU/ml,P <0 .0 5。结论 青少年糖尿病患者中ICA阳性率高于GADA。自身抗体阳性患儿胰岛功能明显低于阴性患儿 ,提示自身抗体阳性患儿胰岛功能损伤更明显。由于ICA、GADA两种抗体的阳性结果常不一致 。
Objective To elucidate whether autoantibodies can be used to predict the intensity of aotoimmune β cell destruction, we determined both C peptide,Insulin and autoantiboties[islet cell antibodies(ICA) and antibodies to glutamic acid decarboxylase(GADA)] in 31 one type diabetic children and adolescents. Methods GADA with enzyme linked immunosorbent assay, ICA with immunoflurescence ,insulin and C peptide with chemiluminescence. Results In diabetic children, the positive rate of ICA was higher than that of GADA (45.2 % vs 38.7 %). Fasting C peptide (0.99±0.36 ng/ml) , postprandial C peptide (2.10±0.99 ng/ml) and postprandial insulin(11.53±7.11 μIU/ml) of autoantibody positive diabetics were lower than those of autoantibody negative diabetics[(fasting C peptide 2.19±1.33 ng/ml),postprandial C peptide(6.18±3.91 ng/ml) and insulin (18.60±10.59 μIU/ml)],P0.05.Conclusions In diabetic children and adolescents, the positive rate of ICA was higher than that of GADA. The islet function of diabetics with positive antibodies was lower than that of diabetics with negative antibodies, indicating that in the former β cell was markedly destruction. The combined determination of GADA and ICA may enhance the sensitivity of diagnosis.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2003年第3期177-178,共2页
Journal of Applied Clinical Pediatrics
