摘要
目的探讨锌转运体8自身抗体（ZnT8A）、谷氨酸脱羧酶抗体（GADA）和酪氨酸磷酸酶抗体2（IA-2A）在1型糖尿病（T1DM）初发病后不同病程的变化。方法60例病程2年（每隔3月复诊）和75例病程5~10年（中位数6.9年）的T1DM患者于初发病和复诊时采集血清,用放免法检测ZnT8A、GADA和IA-2A水平。结果135例T1DM患者初发病时ZnT8A、GADA和IA-2A的检出率分别为35.6%（48/135）,57.0%（77/135）和23.7%（32/135）,三抗体联合检测总阳性率为70.4%（95/135）。病程2年的患者ZnT8A和IA-2A的滴度分别在发病后第15、18个月内下降了42.2%和44.8%,变化显著（P〈0.001）,其后下降不显著（P〉0.05）;GADA则无显著变化（P〉0.05）。75例T1DM患者病程5-10年后,ZnT8A和IA-2A的水平下降了48.2%和54.1%,变化显著（P值均〈0.001）,而GADA则无显著变化（P〉0.05）。结论ZnT8A和IA-2A水平在T1DM发病早期尤其是发病后18个月内随病程延长而显著降低,GADA则不随病程变化。
Objective To explore the dynamic changes of zinc transporter isoform 8 autoantibody（ZnT8A）,glutamic acid decarboxylase antibody（GADA） and protein tyrosine phosphatase antibody（IA-2A） in different disease duration after type 1 diabetes mellitus（T1DM） onset.Methods ZnT8A was detected by radio immunoprecipitation assay,GADA and IA-2A were detected by RIA in serum of 135 T1DM cases from two groups： 60 cases with 2 years duration and monitored every 3 months,75 cases with 5-10 years duration（median 6.9 years）.Results The positive rate of ZnT8A,GADA and IA-2A in T1DM onset cases were 35.6%（48/135）,57.0%（77/135） and 23.7%（32/135）,the positive rate of combination detection of three autoantibodies was 70.4%（95/135）.In cases with 2 years duration,the titers of ZnT8A and IA-2A were declined rapidly 5 and 18 months after onset（P 0.001）,but changed slowly 6 or 9 months later（P0.05）,and titers of GADA showed no significant change.In cases with 5-10 years duration,the titers of ZnT8A and IA-2A dropped by 48.2% and 54.1%（P 0.001）,GADA also showed no significant change（P0.05）.Conclusions The titers of ZnT8A and IA-2A were declined rapidly 18 months after onset,but titers of GADA kept steady after onset.
出处
《中国热带医学》
CAS
2013年第6期735-737,共3页
China Tropical Medicine
基金
深圳市科技计划项目(No.201003305)
关键词
糖尿病
自身抗体
锌转运体8
谷氨酸脱羧酶抗体
酪氨酸磷酸酶抗体2
Type1diabetes mellitus
Autoantibody
Zinc transporter isoform 8
Glutamic acid decarboxylase antibody
Protein tyrosine phosphatase antibody
