摘要
目的 :观察芪丹通脉片 (Qidantongmaitablee,QDTMT)对心肌缺血再灌注损伤 (MIRI)犬的血浆内皮素 (ET) /一氧化氮 (NO)平衡、血栓素B2 (TXB2 ) / 6 酮 前列腺素 (6 keto PGF1α)平衡的影响 .方法 :成年健康杂种犬 2 4只 ,随机分为空白对照组 (Control)、缺血预处理组 (IP)、QDTMT低剂量组(QDTMTL)、QDTMT高剂量组 (QDTMTH) ,均于同等条件下口服给药 (或生理盐水 ) 1wk后 ,采用结扎犬冠状动脉左前降支的方法建立MIRI模型 ,分别测用药前 (T1)、结扎后 2 0min (T2 )、再灌注后 10min (T3) ,再灌注后 30min (T4 ) 4个时间点血浆ET ,NO ,TXB2 与 6 keto PGF1α 的含量 ,分析NO/ET ,6 keto PGF1α/TXB2 的平衡关系 .结果 :与对照组比较 ,QDTMT组能降低血浆ET和TXB2 水平 ,升高NO表达水平 ,提高 6 keto PGF1α的活性 ,升高NO/ET和 6 keto PGF1α/TXB2 比值 (P <0 .0 5 ,P <0 .0 1) ,调节NO/ET及 6 keto PGF1α/TXB2 的平衡关系 ,QDTMTH及QDTMTL与IP比较无显著差异 (P >0 .0 5 ) .结论 :QDTMT能起到与IP相似的保护作用 .其机制与降低血浆ET和TXB2 水平 ,升高NO水平 ,提高 6 keto PGF1α活性 ,调节NO/ET和 6 keto PGF1α/TXB2 的平衡有关 .
AIM: To investigate the effects of Qidantongmai tablet (QDTMT) on the equilibrium between ET and NO and the equilibrium between TXB 2 and 6 keto PGF 1α in the canine model of myocardial ischemia reperfusion injury (MIRI). METHODS: Twenty four adult healthy cross bred canines were randomly assigned to four groups: Control group, ischemia preconditioning group, QDTMT low dosage group (QDTMTL) and QDTMT high dosage group (QDTMTH). Canines in all groups took QDTMT (or NS) orally for a week under the same condition. The model of MIRI was established by ligating levo anterior descending branch of coronary artery. The contents of ET, NO, TXB 2 and 6 keto PGF 1α in plasma were measured at four time points: Pre drug (T 1), 20 min after ligation (T 2), 10 min (T 3) and 30 min (T 4) after reperfusion respectively and the equilibrium between NO / ET and that between 6 keto PGF 1α / TXB2 were analyzed. RESULTS: Compared with the control group, QDTMTL and QDTMTH reduced the level of ET and TXB 2, promoted the expression level of NO, increased the activity of 6 keto PGF 1α , and regulated the equilibrium between NO and ET and that between 6 keto PGF 1α and TXB 2 ( P<0.05, P <0.01). There is no significant difference between QDTMT groups and the ischemia preconditioning group( P >0.05). CONCLUSION: Just as ischemia preconditioning, QDTMT has good protective effects on MIRI. The mechanism of protection relates to its function of decreasing the level of ET and TXB 2, promoting the expression level of No, increasing the activity of 6 keto PGF 1α , and regulating the equilibrium between NO and ET and the equilibrium between 6 keto PGF 1α and TXB 2.
出处
《第四军医大学学报》
北大核心
2003年第5期391-393,共3页
Journal of the Fourth Military Medical University
基金
全军新药开发课题 (后卫中医字第 3号 )
