摘要
目的研究中药护心灵对实验性心肌缺血大鼠缺血复灌注后内皮源性因子内皮素(ET),血栓素B2(TXB2),一氧化氮(NO),6酮前列腺素F1a(6-Keto-PGF1a)及缺血修饰性白蛋白(IMA)的影响及意义.方法40只Wistar大鼠随机分为假手术组、生理盐水组、复方丹参组和护心灵组,每组10只,连续10d灌胃给药后,结扎除假手术组外大鼠的左冠状动脉主干,建立实验性心肌缺血动物模型,并对大鼠缺血复灌注后的内皮源性因子ET,TXB2,NO,6-Keto-PGF1a及IMA的含量进行检测比较.结果实验性心肌缺血大鼠缺血复灌注后生理盐水组、复方丹参组和护心灵组ET,TXB2,TXB2/6-Keto-PGF1a比值及IMA显著高于假手术组,NO及6-Keto-PGF1a显著低于假手术组(P<0.05),复方丹参组和护心灵组与生理盐水组比较则ET,TXB2,TXB2/6-Keto-PGF1a比值及IMA显著低于生理盐水组,NO及6-Keto-PGF1a呈显著高于生理盐水组(P<0.05,P<0.01).结论复方丹参和护心灵均能显著提高急性缺血大鼠内皮源性因子NO及6-Keto-PGF1a水平,降低ET,TXB2水平,对大鼠缺血心肌均具有保护作用,其中以护心灵效果更为显著.
AIM： To investigate the effects and significance of Chinese medicine Huxingling on ET, TXB2, NO, 6-Keto-PGF1a and ischemia-modified albumin （IMA） of experimental cardiac ischemia rats after ischemia reperfusion. METHODS： Forty Wistar rats were divided into 4 groups （ n = 10 in each group） ： sham operation group, sodium chloride group, compound Danshen group and Huxinling group. After 10 d of consecutive intragastric administration, the left main coronary trunk was ligated in all groups except sham operation group to establish experimental cardiac ischemia model to detect and compare the concents of ET, TXB2, NO, 6-Keto-PGF1α and IMA following reperfusion. RESULTS： The concentrations of ET, TXB2, TXB2/6-Keto- PGF1α and IMA of sodium chloride group, compound Danshen group and Huxinling group were all significantly higher than those of sham operation group after ischemia/reperfusion. At the same time , NO and 6-Keto-PGF1α in these 3 groups were significantly lower than those in sham operation group （ P 〈 0. 05 ）. The concentrations of ET, TXB2 , TXB2/6-Keto-PGF1α and IMA of compound Danshen group and Huxinling group were significantly lower than those of sodium chloride group, but ones of NO and 6-Keto- PGF1α were higher than those of sodium chloride group（ P 〈0.05, P 〈0.01 ）. CONCLUSION： Compound Danshen and Huxinling can elevate the concentrations of NO and 6-Keto-PGF1α in acute cardiac ischemia rats and reduce the levels of ET, TXB2. They have protective effects on ischemic cardiac muscle of rats and Huxinling is more effective than compound Danshen.
出处
《第四军医大学学报》
北大核心
2006年第16期1509-1511,共3页
Journal of the Fourth Military Medical University
关键词
护心灵
心肌缺血
内皮源性因子
缺血修饰性白蛋白
Huxinling
myocardial ischemia
endothelium-derived factors
ischemia-modified albumin
