摘要
目的 :探讨大黄多糖 (TMP)对 2 ,4 ,6 三硝基苯磺酸 (TNBS)诱导大鼠结肠炎的治疗作用。方法 :采用大黄多糖组分 (TMP 1)治疗TNBS诱导的大鼠结肠炎 ,以地塞米松 (DX)为阳性药。每天 1次 ,共 5d ,给药结束后 ,尾静脉采血行白细胞计数并分类 ,在乙醚麻醉下腹主动脉采血 ,并处死动物取结肠 ,画取结肠的溃疡面积 ,结肠称重 ,分离肠黏膜以测定SOD ,MPO活性 ;胸腺、脾脏称重。结果 :TMP 1能明显降低结肠炎大鼠的死亡率 ,减轻结肠重量 ,缩小溃疡面积 ,对抗脾脏肿大和胸腺萎缩 ;降低MPO活性 ,升高SOD水平 ,与DX相比 ,其疗效无显著差异 ,也没有DX的免疫抑制副作用。结论 :TMP 1对TNBS诱导的大鼠结肠炎呈现出良好的治疗效果 ,其机制可能与抗炎和免疫调节有关 ;
Objective:To evaluate the effects of Tanguticum Maxim polysaccharide (TMP 1) on TNBS induced colitis in rats. Method:Rats with TNBS/ethanol induced colitis were used and treated with TMP 1 and dexamethasone (DX). Seventy two rats, including animals with TNBS induced colitis, were treated with saline,TMP 1 (100,200,400 mg·kg -1 )and DX. White blood cells were counted on the fifth day and the rats were killed by ether on the sixth day. SOD activity in serum, MPO and SOD activity of colonic tissue were measured. Result:The remarkable effects of TMP 1 at dosage of 200、400 mg·kg -1 on TNBS induced colitis were observed. The ulcerative area was diminished and weight of colon was reduced. White blood cell population was reduced, SOD activity in serum and SOD activity of colon tissue were remarkably increased, and, MPO activity of colonic tissue was reduced. Conclusion: TMP 1 has significant effects on TNBS induced colitis in rats with lower side effects, which suggests the effective component of rhubarb on colitis perhaps is TMP. The mechanism of the actions of TMP may relate to its antiflammation, antioxidation and immunoloregulation.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2003年第3期246-249,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目 (批准号 3 0 10 0 3 2 9)
关键词
2
4
6三硝基苯磺酸
结肠炎
唐古特大黄多糖
地塞米松
2,4,6-trinitrobenzen sulfunic acid
colitis
Tanguticum Maxim polysaccharide(TMP 1)
dexamethasone (DX)
