摘要
目的:研究复方阿魏酸对实验性大鼠脑缺血再灌注损伤的保护作用,并探讨其作用机制。方法:采用大鼠双侧颈总动脉阻断和尾部放血并重复缺血再灌的脑缺血模型。观察复方阿魏酸对大鼠脑组织内皮素-1(ET-1)、降钙素基因相关肽(CGRP)、丙二醛、能量代谢、热休克蛋白70(HSP70)表达及病理组织学的影响。结果:复方阿魏酸可以抑制ET-1、丙二醛及乳酸的过量产生,提高脑组织CGRP、ATP及Na^+-K^+-ATP酶活性。复方阿魏酸的以上作用存在量效关系,其作用优于阳性对照药物丹参。复方阿魏酸治疗组大脑皮层HSP70表达明显增强,大脑缺血损伤程度有减轻。结论:复方阿魏酸对脑缺血再灌注损伤具有保护作用,其机制与改善脑细胞能量代谢、拮抗自由基、改善微循环等多环节作用有关。
Objective: To explore the protective effect and its mechanism of the traditional Chinese medicine complex, ferulic acid compound, on cerebral ischemia reperfusion in rats. Methods :Experimental cerebral ischemia reperfusion in rats was made by occluding the two- vessel and bleeding from tails. To observe the effect of ferulic acid compound on levels of endothelin - 1 (ET - 1) 、 calcitonin gene- related peptide (CGRP) 、 malonaldehyde (MDA) 、 energy metabolism 、 the expression of heat shock protein 70 (HSP70) and histopathological analysis in brain tissue. Results:Ferulic acid compound can decrease the level of ET- 1 、 MDA and lactic acid in brain after cerebral ischemia reperfusion in rats,and also improve the contents of CGRP 、 ATP and Na+ - K+ - ATPase activity.The effect of ferulic acid compound was related to dosages, and its effect was better than the positive salvia miltiorrhiza. Eerulic acid compound can increase the HSP70 expression significantly and alleviate cerebral ischemic changes histopathologically. Conclusion :The effect of ferulic acid compound in many ways may be related to its protection against ischemia reperfusion brain injury.
出处
《重庆医科大学学报》
CAS
CSCD
2003年第1期49-52,共4页
Journal of Chongqing Medical University
关键词
复方阿魏酸
实验
脑缺血
再灌注损伤
Cerebral ischemia reperfusion damage
Ferulic acid compound
Rats
