摘要
目的 研究实验性大鼠脑缺血再灌注损伤时热休克蛋白 70 (HSP70 )的表达及复方阿魏酸对HSP70表达的作用。方法 采用大鼠双侧颈总动脉阻断和尾部放血并重复缺血再灌注的脑缺血模型。应用免疫组织化学方法检测大鼠脑组织HSP70表达 ,并与病理组织学改变进行对照研究。结果 (1)假手术组脑组织无HSP70表达 ,模型组大脑皮层可见HSP70表达 ,复方阿魏酸治疗组大脑皮层HSP70表达明显增强。 (2 )模型组大脑皮层神经细胞呈轻度缺血改变 ,复方阿魏酸治疗组大脑缺血损伤程度有减轻。结论 复方阿魏酸可促使缺血大脑皮层HSP70表达增强 ,减轻皮层神经细胞的缺血损伤 ,对脑缺血再灌注损伤具有保护作用。
Objective To investigate the effect of the traditional Chinese medicine complex, ferulic acid compound, on heat shock protein 70 in rats with cerebral ischemia reperfusion. Methods Experimental cerebral ischemia reperfusion in rats was made by occluding the two vessel and bleeding from tails. The expression of HSP70 was assessed by immunostaining method, histopathological analysis of rat brain was also studied. Results No HSP70 expression was found in non ischemic brain tissue, ferulic acid compound can increase the HSP70 expression significantly compared with that of cerebral ischemia rats, and ferulic acid compound can alleviate cerebral ischemic changes histopathologically. Conclusion Ferulic acid compound increase HSP70 expression and alleviate cerebral ischemic changes histopathologically , it has neuroprotective effect against ischemia reperfusion brain injury.
出处
《重庆医学》
CAS
CSCD
2002年第11期1061-1062,共2页
Chongqing medicine
关键词
脑缺血再灌注损伤
复方阿魏酸
热休克蛋白70
cerebral ischemia reperfusion damage
ferulic acid compound
heat shock protein 70
