摘要
用 6个大豆品种进行了抗生素敏感性试验 ,结果表明 :在培养基中加入 5 0mg/L卡那霉素可显著地降低出愈率。大豆不同品种对卡那霉素的反应在出愈率上表现相似 ,而在体细胞胚胎发生率上存在差异。培养基中含有 40 0mg/L头孢霉素时出愈率显著降低 ,而体细胞胚胎发生率因品种不同而不同。用农杆菌侵染大豆未成熟子叶后在含有头孢霉素的培养基中培养时 ,体细胞胚胎发生率大幅度下降。本试验初步确定了较适合于大豆未成熟子叶抗性选择的卡那霉素浓度和头孢霉素的脱菌浓度。
The experiment of sensitivity on antibiotics was investigated with 6 genotypes of soybean. The results showed that frequency of callus fromation was decreased significantly when the medium was added with 50mg/L kanamycine. The response of soybean genotypes was similar in frequency of callus formation and was different in frequency of somatic embryogenesis. When medium contained 400mg/L cefotaxime, the frequencies of callus formation were dropped, however, the variation of somatic embryogenesis frequencies was different among different genotypes. The frequencies of somatic embryogenesis were declined obviously when immature cotyledon was cultured in medium containing cefotaxime after Agrobacterium-mediated. We provide an ideal selecting press of kanamycine and concentration for inhibiting Agrobacteriumof cefotaxime in immature cotyledon of soybean.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2003年第1期14-17,共4页
Chinese Journal of Oil Crop Sciences
基金
国家植物转基因中试及产业化基地专项基金 (J99-B- 0 0 1)
关键词
大豆
未成熟子叶
体细胞胚胎发生
抗生素
Soybean
Immature cotyledon
Somatic embryogenesis
Antibiotics
