摘要
研究了氨苄青霉素(Amp)、羧苄青霉素(Carb)、头孢霉素(Cef)和卡那霉素(Km)对梨叶片愈伤组织和不定梢诱导的影响。结果表明,Amp对巴梨叶片分化影响较小,当浓度达500mg/L时,其出愈率和不定梢再生率分别为96.48%和56.17%,与对照差异不显著;Cef浓度在50-300mg/L时对巴梨和身不知叶片再生均无显著影响,只有当Cef浓度高于400mg/L时,巴梨的不定梢再生率才受到显著抑制;巴梨和身不知叶片对Carb的反应不相同,低浓度(≤200mg/L)的Carb对巴梨的出愈率和不定梢再生率影响较小,却显著抑制身不知的不定梢再生率,高浓度(≥300mg/L)的Carb不仅极显著抑制巴梨和身不知的出愈率,而且完全抑制了2者的不定梢再生;Km浓度大于10mg/L时,明显抑制愈伤组织的生长,且随着浓度的增加,死亡率逐渐增大,当Km浓度达100mg/L时,巴梨和身不知叶片死亡率达100%和96.30%。
Effects of Ampicillin, Carbenicillin, Cefotaxime and Kanamycin on callus induction and shoot regeneration from the leaves of pear were studied. The results showed that when the concentration of Ampicillin was at 500 mg/L, the rates of callus induction and shoot regeneration from the leaves of Bartlett were 96.48% and 56.17% respectively, which were not significantly different from that of CK. Cefotaxime had no significant effects on the regeneration of the leaves of Bartlett and Shenbuzhi within concentrations at 50-300 mg/L, but shoot regeneration from the leaves of Bartlett was greatly restrained when the concentration of Cefotaxime at more than 400 mg/L. The influence on callus induction and shoot regeneration from the leaves of Bartlett was not great when the concentrations of Carbenicillin were less than 200 mg/L, while not only the callus induction from the leaves of Bartlett and Shenbuzhi was significantly inhibited, but also the redifferentiation of the leaves was thoroughly restrained when the concentrations of Carbenicillin were more than 300 mg/L. Kanamycin obviously inhibited callus induction when its concentrations were more than 10 mg/L, and the rates of death of Bartlett and Shenbuzhi were as high as 100% and 96.30% respectively when the concentration of Kanamycin at 100 mg/L.
出处
《果树学报》
CAS
CSCD
北大核心
2005年第5期454-457,F0003,共5页
Journal of Fruit Science
基金
高等学校全国优秀博士学位论文作者专项基金(200253)教育部优秀青年教师资助计划(2075)农业部"948"项目(2004264)
关键词
梨
抗生素
出愈率
不定梢再生
Pear
Antibiotics
Callus induction
Shoot regeneration
