摘要
用红霉素、头孢唑啉钠、头孢拉定、头孢霉素 (国产和进口 )等 5种抗生素对农杆菌LBA44 0 4进行抑菌试验 ,以头孢霉素的抑菌效果最好。头孢霉素作为抑菌剂用于大豆遗传转化试验时 ,在下胚轴浓度以 30 0mg/L ,在子叶节以 5 0 0mg/L为宜。大豆品种对卡那霉素的反应在出愈率上表现相似 ,在褐化率上表现有些不同。大豆不同外植体对卡那霉素的反应存在较大差异 ,以真叶反应最敏感 ,下胚轴反应最迟钝。在以卡那霉素作为抗性选择标记时 ,选择压力真叶和子叶节以 5 0～ 10 0mg/L为好 ,下胚轴以 10 0～ 2 0 0mg/L为宜。
The experiment of inhibiting Agrobacterium LBA4404 was undertaken with 5 antibiotics (the Erythronycin Base,Cefazolin Sodium,Cefradine,2 kinds of Cefotaximes).Among them,Cefotaxime showed the best effect.When Cefotaxime is used in transformation,the ideal concentration is 300mg/L in hypocotyl and 500mg/L in cotyledon node.The response of soybean varieties to Kanamycin is similar in induction of callus rate and is different in brown rate of callus.Differences of the response of soybean explants to Kanamycin were found.The young leaves are sensitive to Kanamycin,but hypocotyl is not.The ideal selecting pressure of Kanamycin is 50-100mg/L in young leaf and cotyledon node,and100-200mg/L in hypocotyl when Kanamycin is used as selection marker.
出处
《遗传》
CAS
CSCD
北大核心
2001年第4期321-324,共4页
Hereditas（Beijing)
基金
国家转植物基因专项课题 (J99-B -0 0 1)
