摘要
目的:研制尿囊素的皮肤科新制剂,并建立含量测定方法。方法:采用高效液相色谱法测定含量。用C_(18)柱以甲醇-0.03mol/L磷酸二氢钠液(40:60)为流动相,流速0.5ml·min^(-1)检测波长217nm。结果:尿囊素线性范围40.96～204.80μg·ml^(-1),r=0.9994,平均回收率100.7％,RSD为0.5％(n=6)。结论:本品制备简单,含量测定用高效液相色谱法,测定方法专属性强,结果准确可靠,并能很好地控制制剂质量。
Objective: To prepare a new skin preparation of allantoin and establish a method for content determination. Method: The cream base and preparing method were determined according to other cream preparation. The content of allantion is determined by HPLC. G18 column was used with methanol-0. 03 mol/L of NaH2PO4(40:60) as a mobile phase, flow rate was 0. 5 ml·min-1, a detecting wavelength was at 217 nm. Result: The preparation of this cream was simple. The linear range of allantoin was from 40. 96 to 204.80μg·ml-1, r = 0.999 4, the average recovery was 100.7% , RSD = 0.5% (n = 6). Conclusion: This method is simple, special, and accurate for the determination of allantoin by HPLC. It can be used in the quality control of this cream very well.
出处
《中国药师》
CAS
2003年第3期153-154,共2页
China Pharmacist
