摘要
研究高浓度单胺类 (monoamine,MA)递质对神经细胞的毒性作用。观察不同浓度、不同时间多巴胺 (DA)、去甲肾上腺素(NE)和 5 羟色胺 (5 HT)对体外培养神经细胞的毒性 ,并用琼脂糖凝胶电泳、原位末端标记、流式细胞仪进行细胞凋亡的检测。结果显示 ,①高浓度MA引起神经细胞凋亡 ;②给予 80、16 0、32 0 μmol/LDA、NE及 32 0 μmol/L 5 HT处理 2 4h后 ,神经细胞凋亡率明显高于浓度效应对照组 (P <0 0 5 ) ;③给予 30 0 μmol/LDA、NE处理 12、2 4、4 8h后 ,或 30 0 μmol/L 5 HT处理 2 4、4 8h后 ,神经细胞的凋亡率明显高于时间效应对照组 (P <0 0 5 )。提示高浓度MA对体外培养的神经细胞具有细胞毒性 。
To study the toxicity of monoamine in high concentrations to neurons. Neurons were treated in vitro with different doses of dopamine, norepinephrine and serotonin for various lengths of time. The toxicity of these reagents on neurons was observed. Apoptosis of neurons was analyzed by agar electrophoresis, in situ DNA end labeling technique and flow cytometry. The results showed that a high concentration of monoamine could induce apoptosis of the culturing neurons. At 24h after treatment with dopamine or norepinephrine (80, 160 and 320μmol/L) as well as with 320μmol/L serotonin, apoptotic rates of neurons were significantly higher than that of the controls, respectively ( P <0 05). At 12, 24 and 48h following treatment with 300μmol/L dopamine or norepinephrine and at 24 and 48h following 300μmol/L serotonin administration, the apoptotic rates were also significantly higher than that of the controls, respectively ( P <0 05) It is suggested that high dose of monoamine showed a significant cytotoxic effect on cultured neurons and could induce apoptosis of neurons in a dose and time dependent manner.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2003年第1期76-78,I002,共4页
Medical Journal of Chinese People's Liberation Army
基金
广东省科技攻关项目资助课题 (编号 99M0 4 60 66G)
