摘要
目的 :观察硫酸镁对脊髓损伤 (SCI)的保护作用 ,并探讨其可能机制 ,寻求缓解脊髓继发性损伤的新途径。 方法 :成年SD大鼠 4 8只 ,建立脊髓腰膨大半横切模型 ,随机分为对照组、硫酸镁实验组。经处理后检测不同时相损伤部位细胞Ca2 + 浓度、过氧化物歧化酶 (SOD)活性及丙二酰二甲醛 (MDA)浓度 ;4周后进行斜板试验 ,分析功能恢复情况。 结果 :与对照组相比 ,硫酸镁处理组的细胞内Ca2 + 浓度、MDA水平明显下降 ,SOD升高 (P <0 .0 1)。 结论 :硫酸镁对SCI后的脂质过氧化水平和Ca2 + 内流有显著的保护作用 。
Objectives:To investigate the protective effect of magnesium sulfate(MgSO 4) on the injuried spinal cord in SD rats and its possible mechanism so as to seek a novel approch to allievate the secondary spinal cord injury. Methods:All the 48 adult rats were made as hemi section models at the lumbar enlargement and divided into two groups:single spinal cord injury(SCI) group, SCI with MgSO 4 treatment group. The activity of SOD and the concentration of MDA of the spinal cord tissue in the injuried site were measured at 4,8,24 h after reagents were subcutaniously injected 1 h after SCI.Moreover, the concentration of intracellular Ca 2+ at the injury site was assessed at the same time.Functional status was assessed by the slope test at 8 hour, 1 day, 1 week respectively. Results: Activity of SOD was elevated and the level of MDA was reduced significantly in the mammals treated with MgSO 4( P <0.01). The intracellular Ca 2+ was decreased markedly at various time point. Conclusions:Treatment with MgSO 4 can alleviate the lipid peroxidation and calcium influx resulted from secondary damage and thus show a promising future for treatment of SCI.
出处
《医学研究生学报》
CAS
2002年第6期490-492,共3页
Journal of Medical Postgraduates
关键词
硫酸镁
脊髓损伤
保护
Magnesium sulfate
Spinal cord injury
Neuroprotection
