摘要
背景:脊髓损伤后水肿与能量代谢系统障碍可引致一系列放大效应,使损伤的程度更加恶化。基质金属蛋白酶9在颅脑损伤中可引起细胞外基质成分蛋白水解,血脑屏障破坏导致出血及水肿。目的:观察大鼠急性脊髓损伤后基质金属蛋白酶9表达的时空变化,探讨基质金属蛋白酶9在急性脊髓损伤后的作用。设计:完全随机设计。材料:实验在武汉大学医学院病理教研室完成,选择健康雄性SD大鼠40只,随机数分为对照组和模型组。对照组5只大鼠,仅做椎板切除术;模型组35只大鼠,按改良Allen法制作脊髓损伤模型。方法:各组大鼠于术后不同时间点麻醉后,完整取出脊髓组织,切取T8,9脊髓组织,每个组织块随机取连续的3张切片,采用免疫组织化学检测基质金属蛋白酶9表达。每张切片在损伤区(对照组在假手术区)随机采集5个高倍镜(400倍)视野,测定切片的平均吸光度值(A值)。主要观察指标:损伤脊髓中基质金属蛋白酶9的表达。结果:40只大鼠均进入结果分析。模型组急性脊髓损伤后1h可见基质金属蛋白酶9的表达,在一二天达到高峰,7d仍有表达。阳性细胞主要表达在缺血神经元、血管内皮细胞和嗜中性粒细胞。对照组未检测到基质金属蛋白酶9表达。结论:基质金属蛋白酶9在脊髓损伤后早期表达及空间分布特点,提示其在脊髓损伤后组织水肿和炎性细胞浸润中起关键作用。
BACKGROUND：. Edema and systemic disorder of energy metabolism after spinal cord injury can induce a series of amplifying effect, which can deteriorate the injured level. Matrix metalloproteinase-9 （MMP-9） is able to digest the basal lamina and damage the close joint of capillary vessel by proteolysis, which induces blood-brain barrier breakdown and brain edema formation.
OBJECTIVE： To investigate the spatial characteristic of the MMP-9 in acute injured spinal cord so as to investigate the effect of MMP-9 on secondary spinal injury.
DESIGN： A completely randomized study.
MATERIALS： The study was completed in the Department of Pathology, Medical College of Wuhan University. Totally 40 healthy male SpragueDawley rats were divided randomly into control group and model group. Five rats in control group were treated with laminectomy.Other 35 rats in model group was used to establish model of spinal cord injury.
METHODS： After anesthetized, complete spinal cord tissue was taken out. Tissue of T8,9 spinal cord was cut out and every 3 serial sections of each tissue were taken out randomly for immunochemical analysis of MMP-9 expression. Spinal cord tissue at injured segment was taken with 5 rats at each time point. Five visual fields （×400） of every slice were gathered randomly in injured area （sham-operation region in control group）, average absorbency was determined then.
MAIN OUTCOME MEASURES： Expression of MMP-9 in the acute injured tissue of spinal cord.
RESULTS： Totally 40 rats entered the final analysis. The expression of MMP-9 was observed 1 hour in model group with the peak time of 1 to 2 days and also seen on 7 days. The MMP-9 positive cells were mainly expressed on ischemic neuron, vascular entlothelial cells and neutropililic granuloeyte. MMP-9 was not assayed in the control group.
CONCLUSION： The early expression of MMP-9 and the characteristics of spatial distribution after spinal cord injury play a key role in tissue edema and inflammatory cell infiltration.
出处
《中国临床康复》
CSCD
北大核心
2006年第4期180-181,共2页
Chinese Journal of Clinical Rehabilitation
基金
湖北省卫生厅资助项目(W99001)~~
