摘要
植物体内所含的蛋白质、酚类、单宁、色素及多糖等物质影响Tag酶的聚合效率,是使PCR扩增反应失败的主要因素。本研究以针茅属植物为材料,对CTAB法、SDS法、简易提取法和高盐沉淀法提取的针茅DNA进行了比较研究,结果表明:CTAB法适合于针茅属植物基因组DNA的提取,采用这种方法提取的DNA能很好的用于PCR扩增。
High content of phenolic terpenoids,tannin,pigments,polysaccharide,etc.usually effect the activity of Taq and lead to PCR reaction failed.Plant material of Stipa were used for the compared of genome DNA isolation procedure.CTAB,SDS,Rapid one-tube and ion precipitation methods were used for genome DNA isolation.The results suggested that CTAB procedure was suitable for Stipa genome DNA extraction and the DNA can be used for PCR.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2002年第4期32-35,共4页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
国家自然科学基金项目(30060015)
内蒙古自然基金资助项目(20001303)
