摘要
以新鲜天麻块茎为试验材料,采用改良CTAB法提取天麻基因组DNA用于遗传分析和分子标记辅助育种。结果表明,改良CTAB法提取的天麻基因组DNA产量高、纯度高;PCR扩增条带清晰、明亮,无杂带和脱尾;能满足AFLP和SSR的分析要求。说明改良CTAB法能获得高质量天麻基因组DNA。
In this paper, the modified CTAB method was used to extract the genomic DNA of Gastrodia elata Bl for genetic analysis and molecular marker-assisted breeding. The results showed that high quality genomic DNA with less impurity was extracted by the modified CTAB method. Amplified bands were clear and stable without zone tailing and other non-specific bands. The DNA samples extracted by the modified CTAB method was ideal and suitable for further AFLP and SSR analysis. Thus the modified CTAB method can be used to get high quality genomic DNA of Gastrodia elata Bl.
出处
《广东农业科学》
CAS
CSCD
北大核心
2009年第8期153-155,共3页
Guangdong Agricultural Sciences
基金
国家科技攻关项目(2004BA721A33)
贵州省中药专项(黔科中药专字[2003]51号)
