摘要
天麻Gastrodiaelata组织内含有大量的糖类、酚类等次生代谢物质 ,用常规的CTAB(十六烷基溴化铵 )法或SDS(十二烷基硫酸钠 )法难以获得高质量的DNA模板。为获得高质量的DNA ,笔者通过对兰科植物天麻基因组DNA几种提取方法以及天麻不同部位所得的DNA质量进行分析。结果表明 ,SDS -CTAB法是较理想的提取方法 ,能满足普通PCR的需要。天麻不同部位DNA提取的结果表明 。
Gastrodia elata B1 is rich in polysaccharides,phenol and other secondary metabolites.It is difficult to obtain high quality of genomic DNA from tissues by conventional methods such as CTAB(cety-trimethylammonium bromide)method and SDS(sodium dodecyl sulfate)method.Based on the research results of comparative analysis of several extraction methods for total genomic DNA of Gastrodia elata B1 in Orchidaceae, SDS-CTAB method was found to be optimal to produce high quality DNA templates which were suitable for polymerase chain reaction(PCR). The results also showed that among the different tissues of Gastrodia elata B1, the young floral shoots were the best position to extract good genomic DNA.
出处
《山地农业生物学报》
2004年第5期422-425,共4页
Journal of Mountain Agriculture and Biology
