摘要
利用８种ＲＡＰＤ引物（ＯＰＨ－２、ＯＰＨ－３、ＯＰＨ－５、ＯＰＨ－１２、ＯＰＨ－１５、ＯＰＨ－１６、ＯＰＨ－１８和ＯＰＨ－２０）对大豆属的２１份植物材料，其中包括Ｇｌｙｃｉｎｅ亚属的１０个种和Ｓｏｊａ亚属的３个种，进行了基因组指纹图谱构建。通过对获得的基因组指纹图谱的量化分析，利用Ｕｎｗｅｉｇｈｔｅｄｐａｉｒｇｒｏｕｐｗｉｔｈｍａｔｈｅｍａｔｉｃａｖｅｒａｇｅ（ＵＰＧＭＡ）对大豆属中的各个种进行了亲缘关系重建。重建的亲缘关系表明：Ｇ．ｔｏｍｅｎｔｅｌｌａ种中存在３种不同的进化类型，其分化距离已大于某些种种间的分化距离，它们可能是被形态遮蔽的３个种。Ｓｏｊａ亚属内３个种的分化关系同前人的研究推断相同，其亲缘关系很近，这一结果支持将这３个种归并为一个种的观点。但是重建的亲缘关系未能显示出大豆属两个亚属的划分格局。
Genomic fingerprints of 21 accessions of genus Glycine,belonging to 10 species of subgenus Glycine and 3 species of subgenus Soja,have been constructed with 8 RAPD primers (OPH-2, OPH-3, OPH-5,OPH-12, OPH-15, OPH-16,OPH-18, and OPH-20). By the analysis of the data of the genomic fingerprints,the genetic relationship of the accessions was reconstructed with UMPGA. The reconstructed phylogeny showed that there existed three types in G. tomentella and the genetic distance between them had been more than those between some other species. They might be three cryptic species. The divergence of three species in subgenus Soja was as primary reports. Their genetic relationship were close. The result supports the opinion that the three species should be considered as one.The reconstructed dendrogram of genus Glycine did not showa clear division between two the subgenera.
基金
中国科学院重大项目
关键词
大豆属
亲缘关系
RAPD
RAPD-fingerprinting, Genus Glycine, Phylogeny
