摘要
对溶菌酶降解壳聚糖的过程进行了研究。探讨了降解过程的粘度变化、温度、pH值、反应时间、酶浓度、壳聚糖浓度以及壳聚糖的脱乙酰化度对酶促反应速度的影响。实验结果表明,以壳聚糖为底物的溶菌酶的催化是属米氏酶的特征,该酶促反应的最适宜温度为50℃,适宜pH值是6.0左右,壳聚糖的脱乙酰度越低降解速度越快。产品经高效液相色谱测定相对分子质量低于1万,相对分子质量组分集中,水溶性良好。
The effects of reaction temperature, pH, substrate and enzyme concentration, deacetylation degree of chitosan on the hydrolytic velocity of chitosan by Lysozyme were investigated. The results showed that the specificity of catalyst obey the dynamics of michae-lis-Menten. The optimum temperature and pH were 50℃ and 6. 0 respectively, the reaction velocity was reduced while the deacetylation degree of chitosan was raised. The mean molecular weight of low molecular chitosan has been determined by high performance liquid chro-matography method, the mean molecular weight was 8328.
出处
《中国海洋药物》
CAS
CSCD
2002年第6期25-28,共4页
Chinese Journal of Marine Drugs
