摘要
目的 了解对-壬基酚(p-NP)的体外发育毒性特点,为揭示对-壬基酚的发育毒性作用机制提供实验依据。方法 采用微团培养观察不同浓度的对-壬基酚(0～17.5 mg/L)对体外培养的Wistar大鼠胚胎肢芽细胞增殖和分化的影响。结果 对-壬基酚对大鼠胚胎肢芽细胞的增殖和分化均有抑制作用,表现为染毒组肢芽细胞集落形成率降低,细胞毒性试验吸光度值下降,并有明显的剂量-反应关系。对-壬基酚对肢芽细胞的50％增殖抑制浓度(IP_(50))和50％分化抑制浓度(IP_(50))分别为15.85 mg/L和11.86 mg/L,IP_(50)/ID_(50)=1.34。结论 按照Renault等人推荐的体外致畸分类标准,对-壬基酚属于阳性致畸物,对大鼠胚胎肢芽细胞的分化和增殖均有特异性抑制作用。
Objective To investigate the characteristic of developmental toxicity and its mechanism of p-nonylphenol (p-NP) in vitro. Methods The micromass culture of Wistar rat embryo limb bud cells was used to evaluate the cytotoxicity of p-NP(0-17. 5 mg/L) and its effect on embryonic chondrogensis in vitro. Results p-NP inhibited both proliferation and
differentiation of rat limb bud cells in vitro. High levels of p-NP appeared to be cytotoxic to Wistar rat embryo limb bud cells in culture and inhibited the clone formation with dose-response relationship. The concentration of p-NP for IP50 and ID50 were 15. 85 mg/L and 11. 86 mg/L respectively. ' Conclusions According to Renaults' teratogenic criteria, p-NP belonged to a positive teratogen. And the data suggested that the specific inhibition of cell proliferation and differentiation might be one of the mechanisms of developmental toxicity of p-NP.
基金
国家自然科学基金(No.30030120)
国家"973"项目(No.G1999055904)资助
