摘要
用大鼠胚胎神经细胞微团培养法，研究了镉、铬、铝、铅、镍、钡、锆、钯八种金属化合物的体外致畸作用，并提出了金属化合物的体外细胞致畸结果判断标准。结果表明，八种金属化合物都有较明显的细胞毒性，都能抑制胚胎神经细胞分化。镉、铬、铝、铅、镍五种化合物对神经细胞的半数分化抑制浓度（ＩＣｄ５０）均小于１μｇ／ｍｌ，半数存活抑制浓度（ＩＣｖ５０）与ＩＣｄ５０比值（Ｖ／Ｄ）均大于６．０，判断为体外细胞致畸试验阳性。钡、锆、钯三种金属化合物Ｖ／Ｄ值近于１，认为其对细胞分化的抑制作用是由细胞毒性所致。
The micromass rat embryo midbrain culture were used in an effort to determine the teratologenicity of eight metallic compounds [Cd(Ac)_2,CrCl_2,Al_2(SO_4)_3,Pb(Ac)_2,NiNO_3 ,BaCl_2 ’ZtSO_4 ,PdCl_2],The criteria were proposed for determination of teratogenicity of metallic compounds in vitro. All of the compounds have obvious cytotoxicity and can inhibit differentiation(foci formation)of the cultrued neurons.The IC50 values for foci formation(ICd50)of five metallic compounds [Cd(Ac)_2,CrCl_2,Al_2(SO_4)_3,Pb(Ac)_2,NiNO_3] are less than 1.0μg/ml.These ICd_(50) values were compared to the IC50 values for cell survival(ICv50).When the two sets of IC50s differed by more than a factor 6 that compound was considered as a teratogen(T).For BaCl2, PbCl2 and ZrSO4, the difference in IC50 values for formation and cell survival was smaller.i.c. equal of to 1.0 aproximately,It can been considered that the inhibition of differentiation induced these com pounds result from the cytotoxicity.Consenquently these substances were classified as non teratogens(NT)in vitro.
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1994年第1期14-16,共3页
Journal of Environment and Health
基金
国家自然科学基金
关键词
微团培养
体外致畸
细胞分化
金属
Micromass cultrue Teratogenicity in vitro Cell differentiation
