摘要
采用盐酸胍抽提,丙酮分级沉淀,超滤等步骤从牛软骨中提取出软骨抗肿瘤组分(Cartilage antitumor component,CATC)。利用[~3H]-TdR掺入及肿瘤干细胞测定等方法研究CATC对肿瘤的抑制效应,结果显示:(1)CATC可明显抑制Hela细胞及QGY_(7703)细胞的DNA合成,而对正常人皮肤成纤维细胞则有促进作用;(2)CATC对两种肿瘤干细胞均有明显抑制,其中对Hela细胞抑制作用较强;(3)CATC对小牛主动脉血管内皮细胞有明显抑制作用。上述结果说明CATC既能直接抑制肿瘤细胞生长,又能抑制血管生成切断肿瘤的营养供应,具有潜在的肿瘤治疗价值。
Cartilage antuumor component (CATC) was isolated from a 1 mol/L guanidine hydrochloride extract of bovine cartilage by acetone fractioned precipitation and superfiltration. Using human skin fibroblasts as a normal control, it was demonstrated that CATC inhibited the DNA synthesis of Hela, QGY7703 tumor cell lines and bovine artery endothelial cells, but accelerated the normal cells, when the concentration was below 1250 μg/ml. At the concentration of 5 000 μg/ml, CATC inhibited the two cell lines. With human tumor stem cell assay, CATC inhibited the stem cell growth of Hela and QGY7703 cell lines. These suggest that CATC has the effects of inhibiting angiogenesis and tumor cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1992年第6期551-555,共5页
Academic Journal of Second Military Medical University
关键词
软骨
肿瘤
抗癌药
cartilage antilumor component
angiogenesis inhibiting factor
tumor cell inhibiting fac- tor
tumor therapy
