摘要
小牛软骨经盐酸胍抽提、丙酮分级沉淀及膜超滤等步骤获得了软骨抗肿瘤制剂（Ｃａｒｔｉｔａｇｅ Ａｎｔｉｕｍｏｒ Ｐｒｅｐａｒａｔｉｏｎ，ＣＡＴＰ）。利用＾３Ｈ－ＴｄＲ参入，测定肿瘤干细胞及细胞死亡率等方法研究了ＣＡＴＰ对肿瘤细胞及血管内皮细胞的换制效应，并对小鼠移植性肿瘤进行了抗肿瘤作用的研究。结果表明：（１）合适浓度的ＣＡＴＰ可显著地抑制肿瘤细胞和血管内皮细胞的生长，而不抑制正常细胞；
Cartilage antitumor preparation (CATP) was isolated from a guanidine hydrochloride extract of bovine cartilage by acetone fractioned precipitation and ultrafiltration. By the methods of 3H-TdR incorporation, tumor stem cell assay and the determination of mortality of cell, the effect of CATP on the tumor cell lines and bovine artery endothelial cells were investigated and the antitumor effect of CATP was observed by the experiment of transplanation of tumor into mice. The results were as follows; (1) CATP had the effects of inhibiting angiogenesis and tumor cells; (2) CATP markedly inhibited both the growth of sc solid tumor of sarcoma 180 and that of Lewis lung carcinoma in the mice, inhibition rate was 50%-60% ; (3) Thymus weight of treated group obviously increased in comparison with control group, indicating that CATP had immunoregulatory effect. The experimental results furnished evidence in order to apply to clinic.
出处
《中国生化药物杂志》
CAS
CSCD
1996年第4期150-154,共5页
Chinese Journal of Biochemical Pharmaceutics
基金
国家自然科学基金资助项目
关键词
软骨
抗肿瘤制剂
血管生成抑制
抗肿瘤活性
Cartilage, Antitumor preparation, Anti-angiogenesis, Antitumor activation, Experimental tumor therapy
