摘要
目的 观察苦参碱对人胶质瘤细胞株BT32 5体外增殖的影响 .方法 应用MTT比色法检测不同浓度苦参碱对BT32 5细胞的增殖抑制 ,应用流式细胞仪观察苦参碱对BT32 5细胞周期的影响 ,采用透射电镜观察瘤细胞形态学改变 .结果 苦参碱对BT32 5细胞增殖抑制作用呈剂量依赖性 ,当浓度为 0 5g·L- 1 时 ,对BT32 5增殖的抑制率最大 ,达到 (32 1±1 1) % .流式细胞仪分析显示 ,用 0 5g·L- 1 苦参碱培养 72h ,BT32 5细胞出现典型凋亡峰 ,凋亡细胞占细胞总数 2 3 9% .透射电镜观察 ,发现有凋亡细胞存在 ,表现为细胞皱缩 ,染色质边集 .结论 苦参碱对人胶质瘤细胞系BT32 5的增殖具有明显的抑制作用 ,并能诱导其发生凋亡 .
AIMTo investigate the effects of matrine on the proliferation of human glioma cell line BT325. METHODS MTT was used to measure the levels of the proliferation of BT325 cultured with matrine in different concentrations. The effects of matrine on cell cycle of BT325 were observed by FCM. The morphological change of BT325 cell was detected by electronic microscopy. RESULTS The proliferation of BT325 was obviously inhibited by matrine in a dose dependent manner. The inhibitory rate came to the peak at (32 1±1 1)%, when cultured with matrine at 0 5 g·L -1 . The outcome of FCM showed that the apoptotic cell rate was 23 9%. The morphological changes of apoptosis were also found by electronic microscopy. CONCLUSION Matrine can inhibit the proliferation of BT325 and induce the apoptosis of the cells.
出处
《第四军医大学学报》
北大核心
2002年第23期2152-2154,共3页
Journal of the Fourth Military Medical University
