摘要
研究 c- m yc在化疗药物顺铂诱导肿瘤细胞凋亡中的作用。在原有已构建好 c- myc反义表达载体 pc DNA3- MYC并成功转染 BT32 5 细胞的基础上 ,用顺铂诱导细胞凋亡。采用 TUNEL技术并从光镜、电镜、荧光显微镜及激光共聚焦显微镜水平观察 BT32 5 细胞凋亡的形态学特征 ;流式细胞仪检测实验组 (即 pc DNA3- MYC转染的细胞 )及对照组 (包括未转染或仅转染空载体 pc DNA 3的细胞 )凋亡率的异同。结果显示 :与转染空载体 pc DNA3及未转染组的细胞相比 ,在转染 pc DNA 3-MYC的 BT32 5 细胞中 ,顺铂诱导的细胞凋亡作用明显受阻 ,结果表明 :肿瘤细胞内异常表达的 c- myc在顺铂诱导肿瘤细胞凋亡的过程中有重要作用。
The purpose of this study was to examine the effect of c- myc expression on cisplatin- induced- apoptosis.A recombinanteukaryotic expression vectorof c- myc antisense( pc DNA3- MYC) was previously constructed and used to transfect human glioma cell line BT3 2 5.Cell apoptosis was induced by cisplatin. The morphological changes of apoptotic BT3 2 5cells were examined with light microscope,electron microscope,fluorescence microscope and laser confocal scanning microscopy.Then we detected the difference ofapoptotic cell percentage among the experimental and control cell groups.Compared with that in the control cell groups,the cisplatin- induced- apoptosis was greatly blocked in the pc DNA3- MYC transfected cell group.The deregulated expression of c- myc in tumors cells may play an important role in the anti- cancer drug inducing apoptosis.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2000年第3期322-325,共4页
Chinese Journal of Histochemistry and Cytochemistry
