摘要
本试验旨在探究碱韭黄酮(APTF)对呕吐毒素(DON)致鸡胚腺胃炎的保护作用及其机制。选取0胚龄健康海兰褐种蛋,设置对照组(CON组)、DON组(每蛋10μg DON蛋)、APTF组(每蛋150μg APTF)和DON+APTF组(每蛋10μg DON+150μg APTF),9胚龄时通过尿囊腔注射建立模型,15胚龄时检测腺胃组织病理学变化、DON残留量、炎症因子水平及焦亡相关蛋白表达。结果表明:1)DON暴露显著降低腺胃脏器指数(P<0.05),诱导黏膜上皮脱落、腺体萎缩及炎性细胞浸润,而APTF干预改善上述病理损伤;2)高效液相色谱-串联质谱(HPLC-MS/MS)法检测表明,APTF未显著影响腺胃DON残留量(P>0.05),提示其作用靶点为下游毒性通路;3)qPCR和Western blot分析显示,DON显著上调腺胃白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、环磷酸鸟苷-磷酸腺苷合成酶(cGAS)和干扰素基因刺激因子(STING)mRNA相对表达量(P<0.05)以及Toll样受体4(TLR4)、NOD样受体热蛋白结构域相关蛋白3(NLRP3)、剪切型胱天蛋白酶-3(cleaved Caspase-3)和gasdermin家族成员E(GSDME)蛋白相对表达量(P<0.05),而APTF干预可显著抑制这些分子变化(P<0.05)。由此可见,APTF通过阻断TLR4/核因子-κB(NF-κB)/NLRP3炎症信号通路及胱天蛋白酶-3(Caspase-3)/GSDME依赖性焦亡途径,缓解DON诱导的腺胃炎,这为开发靶向焦亡通路的霉菌毒素解毒剂提供了理论依据。
This study investigated the protective effects and underlying mechanisms of Allium polyrhizum Turcz.ex Regel flavonoids(APTF)against deoxynivalenol(DON)-induced proventriculitis in chicken embryos.Healthy Hy-Line brown fertilized eggs at 0 day of embryo age were randomly allocated into four groups as control group(CON group),DON group(10μg DON per egg),APTF group(150μg APTF per egg),and DON+APTF group(10μg DON+150μg APTF per egg).The model was established via allantoic cavity injection at 9 days of embryo age,with evaluations including glandular stomach histopathology,DON residues,inflammatory factor levels,and pyroptosis-related protein expression at 15 days of embryo age.Key findings demonstrated:1)DON exposure significantly reduced the organ index of glandular stomach(P<0.05),inducing mucosal epithelial shedding,glandular atrophy,and inflammatory cell infiltration,while APTF intervention markedly alleviated these pathological damages.2)High performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)analysis indicated that APTF did not significantly affect DON residues in glandular stomach(P>0.05),suggesting that its mechanism targets were downstream toxicity pathways.3)qPCR and Western blot analyses revealed that DON significantly upregulated the mRNA relative expression levels of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),cyclic GMP-AMP synthase(cGAS),and stimulator of interferon genes(STING)and protein relative expression levels of Toll-like receptor 4(TLR4),NOD-like receptor thermal protein domain associated protein 3(NLRP3),cleaved Caspase-3,and gasdermin E(GSDME)(P<0.05),whereas APTF intervention significantly suppressed these molecular changes(P<0.05).Thus,it can be concluded that APTF alleviates DON-induced proventriculitis by inhibiting TLR4/nuclear factor-κB(NF-κB)/NLRP3 inflammatory signaling pathway and Caspase-3/GSDME-dependent pyroptosis pathway,providing a theoretical basis for developing targeted pyroptosis inhibitors as detoxifying agents against mycotoxins.
作者
赵鹏
高健
郑豪
赵彩权
白力格
刘杰
卢玲
高雪峰
董贵成
陈甫
董文轩
ZHAO Peng;GAO Jian;ZHENG Hao;ZHAO Caiquan;BAI Lige;LIU Jie;LU Ling;GAO Xuefeng;DONG Guicheng;CHEN Fu;DONG Wenxuan(School of Ecological and Environment,Baotou Teachers’College,Inner Mongolia University of Science and Technology,Baotou 014030,China;College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China)
出处
《动物营养学报》
北大核心
2026年第1期621-633,共13页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(32373078)
内蒙古自治区中药材现代农牧业产业技术体系项目(IMARS-11)
青岛市自然科学基金项目(24-4-4-zrjj-37-jch)
山东省自然科学基金(ZR2021MC150,ZR2022QC243)
山东省家禽产业技术体系项目(SDAIT-11-07)
内蒙古自治区第十二批“草原英才”创新创业人才团队(内人才发[2023]1号)283
2022年度“草原英才”滚动支持人才项目(内组通字[2022]15号)139
内蒙古自治区人才开发基金2022年度(总第二十二批)高层次人才个人项目(内人社办发[2022]110号)66
内蒙古自治区自然科学基金项目(2025MS03023)。
关键词
碱韭黄酮
呕吐毒素
鸡胚
腺胃炎
焦亡
Allium polyrhizum Turcz.ex Regel flavonoids
deoxynivalenol
chicken embryos
proventriculitis
pyroptosis
