摘要
为探究益生菌制剂对特应性皮炎症状的影响,本研究选择了与皮炎相关免疫关联的多种潜在益生菌以及包括低聚糖在内的多种植物化合物,评估了混合益生菌,混合植化物和混合益生菌制剂(上述两种混合益生菌和混合植化物的组合)对特应性皮炎(Atopic Dermatitis,AD)的缓解作用,以探究益生菌和植化物复配的协同增效作用。结果表明,相比混菌组和混合植化物组,混合益生菌制剂对AD症状的缓解效果最为突出,表现为对AD样皮损中炎症细胞浸润的更显著抑制(P<0.001),血清总免疫球蛋白E和背部皮肤组织中炎症细胞因子水平的更大幅度降低,干扰素-γ水平的更大幅度升高。此外,肠道菌群分析结果表明,与AD模型组小鼠对比,混合益生菌制剂干预在属水平上提高了Akkermansia、Bifidobacterium、Turicibacter和Faecalibaculu等有益菌的相对丰度,在OTU水平上显著提高了Bifidobacterium animalis等的相对丰度(P<0.05)。肠道菌群互作网络和关联性分析结果表明,混合益生菌制剂干预富集的肠道菌与免疫炎症因子的改变显著相关(P<0.05)。综上,相比单独的混合益生菌和混合植化物,混合益生菌制剂显示出更优的皮炎缓解作用,实现了协同增效,有助于通过调节免疫炎症反应和肠道菌群来缓解AD。
To investigate the effects of probiotic formulation on symptoms of atopic dermatitis(AD),probiotic strains(have been reported to be associated with immune responses in dermatitis),as well as plant-derived compounds(including fructooligosaccharides)were selected based on preliminary experimental results and related reports.To investigate the synergistic enhancement based combinatorial treatment strategies for probiotics and plant-derived compounds,the effects of mixed probiotics,mixed plant-derived compounds,and a probiotic-phytochemical synbiotic formulation(a combination of the aforementioned mixed probiotics and mixed plant-derived compounds)on AD were assessed.The results indicated that compared with the mixed probiotic and mixed plant compound treatments,the probiotic–phytochemical synbiotic formulation contributed to a more pronounced alleviation of AD symptoms,characterized by a significantly stronger inhibition of inflammatory cell infiltration in AD-like skin lesions(P<0.001),a more substantial reduction in total levels of immunoglobulin E in serum and content of inflammatory cytokines in dorsal skin tissues,and a more significant elevation of interferon-γ.Additionally,analyses of the gut microbiota revealed that compared with the AD model group,intervention using the probiotic-phytochemical synbiotic formulation promoted increases in the relative abundances of beneficial bacterial genera,including Akkermansia,Bifidobacterium,Turicibacter,and Faecalibaculum,whereas at the OTU level,there was a significant elevation in the relative abundance of Bifidobacterium animalis(P<0.05).Furthermore,microbe-microbe network and correlation analyses revealed a significant association between the enriched gut bacterial composition following intervention with the probiotic-phytochemical synbiotic formulation and the levels of immune-inflammatory factors(P<0.05).In summary,compared with mixed probiotics and plant-derived compounds,a probiotic-phytochemical synbiotic formulation was more effective in alleviating AD,conferring measurable synergistic effects and contributing to the amelioration of AD via routes involving the modulation of both immune responses and the gut microbiota.
作者
喻喜华
王一
李刘若兰
王宁
肖越
王鸿超
YU Xihua;WANG Yi;LI Liuruolan;WANG Ning;XIAO Yue;WANG Hongchao(Sinopharm Xingsha Pharmaceuticals(Xiamen)Co.,Ltd.,Xiamen 361026,China;Xiamen Key Laboratory of Maternal and Infant Health and Nutrition Products,Xiamen 361026,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
出处
《食品工业科技》
北大核心
2026年第1期433-442,共10页
Science and Technology of Food Industry
