摘要
虾壳是目前几丁质的最主要来源,对虾壳脱灰分是制备几丁质的关键步骤。本研究从废弃虾塘中分离到两株产酸菌株GXUN-XS3和GXUN-XS8,运用形态学及分子生物学方法对其进行鉴定,通过单因素实验及响应面试验优化菌株的最优发酵工艺,利用SEM对发酵产物进行表征分析。结果表明,GXUN-XS3和GXUN-XS8分别为粪肠球菌和海岸微小杆菌。优化后GXUN-XS3在含2%虾壳粉、3%葡萄糖、1%氯化钠的培养基中以温度30.5℃、初始pH5.6、接种量3.3%条件下发酵3 d,虾壳脱灰分率达到96.09%,较优化前提高6.29%。GXUN-XS8在含2%虾壳粉、2%麦芽糖、1%氯化钠的培养基中以温度30.7℃、初始pH5.7、接种量3.9%条件下发酵4 d,虾壳脱灰分率达到95.73%,较优化前提高6.17%。SEM分析发现,产物的微观结构均呈现出纤维状态并带有孔隙,与酸碱法制备的工业几丁质相比,其纤维组织更加完整。本研究为微生物发酵法降解虾壳废弃物提供了菌株资源和有益参考。
Shrimp shells are currently the primary source of chitin,and demineralization of shrimp shells is a key step in the preparation of chitin.In this study,two acid-producing strains,GXUN-XS3 and GXUN-XS8,were isolated from abandoned shrimp ponds.They were identified through morphological and molecular methods.The optimal fermentation conditions were optimized using single-factor and response surface experiments.SEM analysis was used to characterize the fermentation products.The results showed that the isolated strains GXUN-XS3 and GXUN-XS8 were identified as Enterococcus faecalis and Exiguobacterium aestuarii,respectively.The optimized GXUN-XS3 was fermented in a medium containing 2%shrimp shell powder,3%glucose,and 1%sodium chloride at a temperature of 30.5℃,an initial pH of 5.6,and an inoculum size of 3.3% for 3 days.The demineralization of shrimp shell reached 96.09%,which was an increase of 6.29%compared to before optimization.The optimized GXUN-XS8 fermented in a medium containing 2% shrimp shell powder,2% maltose,and 1%sodium chloride at a temperature of 30.7℃,an initial pH of 5.7,and an inoculum size of 3.9%for 4 days.The demineralization of shrimp shell reached 95.73%,which was an increase of 6.17%compared to before optimization.SEM analysis revealed that the products had a fibrous structure with pores,more intact than industrial chitin produced by acid-alkali methods.This study provides microbial resources and references for the microbial fermentation degradation of shrimp shell waste.
作者
潘小卓
谢勇俊
尹凯波
郑子露
王一兵
PAN Xiaozhuo;XIE Yongjun;YIN Kaibo;ZHENG Zilu;WANG Yibing(School of Marine Sciences and Biotechnology,Guangxi Minzu University,Guangxi Key Laboratory for Polysaccharide Materials and Modifications,Nanning 530008,China)
出处
《食品工业科技》
北大核心
2026年第1期279-288,共10页
Science and Technology of Food Industry
基金
广西重点研发计划项目(桂科AB21196019)
广西科技重大专项(桂科AA18242026)
广西民族大学自治区级大学生创新创业训练计划项目(S202310608172)。
关键词
虾壳
产酸菌
脱灰分
工艺优化
响应面法
shrimp shell
acid-producing bacteria
demineralization
process optimization
response surface methodology
