摘要
试验旨在探究发酵三角风抑制嗜水气单胞菌的最优发酵工艺。在单因素试验的基础上,以对嗜水气单胞菌的抑菌率为评价指标,采用响应面法对发酵时间、接种量和发酵温度进行优化,确定最佳发酵条件。结果显示,最优工艺参数为发酵时间5 d、接种量8%、发酵温度35℃,在此条件下发酵三角风对嗜水气单胞菌抑菌率可达84.86%。研究表明,发酵三角风对嗜水气单胞菌具有明显的抑制作用,显示出作为水产饲料添加剂的开发潜力。
The experiment aims to explore the optimal fermentation process for inhibiting Aeromonas hydrophila through the fermented Hedera nepalensis K.Koch var.sinensis(Tobler)Rehd.Based on single-factor experiments,the antibacterial rate against Aeromonas hydrophila was used as the evaluation index.Response surface methodology was employed to optimize the fermentation process,including fermentation time,inoculation amount,and fermentation temperature.The optimal fermentation conditions were determined.The results showed that the optimal process parameters were fermentation time of 5 days,inoculation amount of 8%,and fermentation temperature of 35℃.Under this condition,the inhibition rate of Aeromonas hydrophila by the fermentation Hedera nepalensis K.Koch var.sinensis(Tobler)Rehd.could reach 84.86%.The study indicates that fermented Hedera nepalensis K.Koch var.sinensis(Tobler)Rehd.exhibits significant antibacterial activity against Aeromonas hydrophila,indicating its potential as an aquatic feed additive.
作者
李泽洋
张丽萍
黄昌林
张雨彤
郭松
LI Zeyang;ZHANG Liping;HUANG Changlin;ZHANG Yutong;GUO Song
出处
《饲料研究》
北大核心
2025年第21期99-103,共5页
Feed Research
基金
广西科技基地与人才专项(项目编号:2021AC19423)
广西高校中青年教师科研基础能力提升项目(项目编号:2023KY0878)
广西科技师范学院壮瑶药品质生物学重点实验室(项目编号:GXKSKYPT2021007)
广西科技师范学院高层次人才启动经费(项目编号:GXKS2020GKY009)
广西科技师范学院青年科研项目(项目编号:GXKS2022QN102)
广西科技师范学院大学生科研基金项目(项目编号:GXKS2024DXS038)
广西科技师范学院大学生创新创业训练计划项目(项目编号:X2023197)。
关键词
发酵三角风
嗜水气单胞菌
抑菌
响应面法
fermentated Hedera nepalensis K.Koch var.sinensis(Tobler)Rehd.
Aeromonas hydrophila
antibacterial
response surface methodology
