摘要
针对氯氟氰菊酯(Cyfluthrin,CL)残留的环境风险,研究采用富集培养法从CL污染的土壤中分离、筛选、鉴定了一株降解菌Rhodococcus sp.LJF-3,并通过Plackett-Burman因素筛选、最陡爬坡试验和Box-Behnken Design响应面分析法优化了其降解条件。在优化后,菌株LJF-3的最佳降解条件是:15 g/L酵母粉,10 g/L葡萄糖,0.5 g/L K_(2)HPO_(4),0.5 g/L KH_(2)PO_(4),1.0 g/L NaCl,0.5 g/L MgSO_(4),培养基体积为70 mL,接种体积分数为6%,培养温度为30℃,pH值为7.0,转速为180 r/min。优化后的菌株LJF-3在42 h内对1000 mg/L CL降解率达87.4%,较未优化前的降解率(38.9%)提高了48.5百分点。研究为CL的微生物降解提供了优良的菌株资源,为CL污染环境的修复提供了技术支持和方法参考。
To tackle the environmental risks posed by Cyfluthrin(CL)residues,this study utilized enrichment culture methods to isolate CL−degrading bacterial strains.CL−contaminated soil served as the enrichment medium,while a Minimal Medium(MMCL)with CL as the sole carbon source was used for screening.This approach led to the isolation of four strains:LJF-1,LJF-2,LJF−3,and LJF-4.Notably,strain LJF−3 demonstrated rapid degradation of 200 mg/L CL,achieving a degradation rate of 38.9%even at 1000 mg/L.Based on 16S rRNA gene sequencing(GenBank accession number PQ803889),strain LJF-3 exhibited 99.93%sequence similarity to Rhodococcus species in the NCBI database.Physiological and biochemical tests yielded positive results for phenylalanine arylamidase,L-lactic acid alkalization,and tyrosine arylamidase,while other tests returned negative results.Consequently,strain LJF-3 was identified as a species of Rhodococcus.To enhance its degradation efficiency,an 11-factor,3-level Plackett-Burman experimental design was employed,identifying culture volume,glucose concentration,and yeast extract concentration as significant factors.Follow-up steepest ascent experiments with five gradient groups determined the optimal conditions:a culture volume of 70 mL,glucose concentration of 10 g/L,and yeast extract concentration of 15 g/L.A Box-Behnken Design Response Surface Methodology(RSM)was subsequently employed to optimize these three factors,with results indicating a well-fitted model(p<O.O01).The optimized conditions for strain LJF-3 included:15 g/L yeast extract,10 g/L glucose,0.5 g/L K_(2)HPO_(4),0.5 g/L KH_(2)PO_(4),1.0 g/L NaCl,0.5 g/L MgSO_(4),a culture volume of 70 mL,6%inoculation volume,incubation at 30℃,pH 7.0,and agitation at 180 r/min.Under these optimized conditions,strain LJF-3 achieved an impressive degradation rate of 87.4%for 1000mg/L CL within 42 hours,representing a 48.5 percentage point increase compared to the unoptimized rate of 38.9%.In summary,the optimization strategy significantly enhanced CL degradation by strain LJF-3.This study provides a valuable microbial resource and technical framework for the remediation of CL pollution,offering insights for future environmental bioremediation applications.
作者
王慧
朱鸿康
姚培林
马正炫
焦同禹
操璟璟
赵文瑞
万安
祖瑶
胡淑宝
WANG Hui;ZHU Hongkang;YAO Peilin;MA Zhengxuan;JIAO Tongyu;CAO Jingjing;ZHAO Weirui;WAN An;ZU Yao;HU Shubao(College of Resources and Environment,Anqing Normal University,Anqing 246133,Anhui,China;Anhui Provincial Key Laboratory of Intelligent Monitoring and Productivity Improvement of Cultivated Land,Anqing 246133,Anhui,China;College of Life Sciences,Anqing Normal University,Anqing 246133,Anhui,China)
出处
《安全与环境学报》
北大核心
2025年第12期4808-4818,共11页
Journal of Safety and Environment
基金
国家自然科学基金项目(41907145)
安徽省教育厅自然科学重点研究项目(2023AH050484,2024AH051113)
安徽省高校数字种植业创新团队项目(2023AH010039)
安庆师范大学大学生创新创业训练计划资助项目(X202510372075,X202510372076)
安庆师范大学校级研究生质量工程项目(X2025kjxy003)
环境科学专业服务安徽省新兴产业项目(2024fwxx028)。
