摘要
该研究以环渤海地区4个不同产地虾酱为研究对象,利用常规检测分析方法及高通量测序技术分析其理化指标及细菌群落结构,基于检测结果进行主坐标分析(PCoA)、层次聚类分析(HCA),采用线性判别分析效应大小(LEfSe)对差异菌群进行分析,并对细菌群落结构与理化指标进行冗余分析(RDA)及相关性分析。结果表明,不同产地虾酱的pH、水分、盐、氨基酸态氮、丙二醛和挥发性盐基氮等理化指标差异明显,其细菌群落的丰富度、多样性具有显著差异(P<0.05)。不同产地的虾酱共检出厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)等7个优势细菌门(相对丰度>1%),四联球菌属(Tetragenococcus)、乳酸杆菌属(Lactobacillus)等15个优势细菌属。PCoA及HCA均能区分不同产地虾酱;结合线性判别分析(LDA)值>2及方差分析P值<0.05,不同产地虾酱之间显著差异细菌类群有一定区别;RDA结果表明,盐含量是影响不同产地虾酱细菌群落的主要理化指标;相关性分析表明,优势细菌属与理化指标之间存在明显相关性,两者均影响不同产地虾酱品质。
Taking shrimp paste samples from 4 different producing areas in the Bohai Rim region as the research objects,the physicochemical indexes and bacterial community structure were analyzed by conventional analytical methods and high-throughput sequencing technology.Based on the detection results,principal coordinate analysis(PCoA)and hierarchical cluster analysis(HCA)were conducted,the differential bacterial community was analyzed by linear discriminant analysis effect size(LEfSe),and redundancy analysis(RDA)and correlation analysis were conducted on the bacterial community structure and physicochemical indexes.The results showed that there were obvious differences in physicochemical indexes such as pH,moisture,salt,amino acid nitrogen,malondialdehyde,and volatile basic nitrogen in shrimp pastes from different producing areas.Moreover,the richness and diversity of bacterial communities showed significant differences(P<0.05).A total of 7 dominant bacterial phyla(relative abundance>1%)such as Firmicutes and Proteobacteria,and 15 dominant bacterial genera such as Tetragenococcus and Lactobacillus were detected in shrimp pastes from different producing areas.Both PCoA and HCA could distinguish shrimp pastes from different producing areas.Combining linear discriminant analysis(LDA)value>2 and variance analysis P value<0.05,there were significant differences in bacterial communities in shrimp paste from different producing areas.RDA results indicated that salt content was the main physicochemical index influencing bacterial communities of shrimp paste from different producing areas.Correlation analysis revealed that there was significant correlation between dominant bacterial genera and physicochemical indexes,both of which affected the quality of shrimp paste from different producing areas.
作者
郭帅
赵佳
GUO Shuai;ZHAO Jia(Department of Biological Science and Technology,Jinzhong University,Jinzhong 030619,China)
出处
《中国酿造》
北大核心
2025年第11期109-115,共7页
China Brewing
基金
山西省功能食品产业体系建设项目(2023 cyjstxl 0-11)。
关键词
环渤海地区
虾酱
细菌群落
理化品质
相关性
Bohai Rim region
shrimp paste
bacterial community
physicochemical quality
correlation
