摘要
Sanger测序的提出给人类破译遗传密码提供了强有力的工具。而近几年飞速发展起来的高通量测序技术无论在成本还是效率上,都对传统测序提出了巨大的挑战。千元人类基因组计划的公布更是加快了测序研发的进程。通过下一代测序技术,科学家不仅能够绘制出各个物种的基因组图谱,还能探讨不同条件下的基因表达差异以及不同物种之间或者物种之内的序列差异,进而为传统生物学以及医学研究开辟新的视角。与此同时,随着各大测序平台的不断改进,普通实验室都有能力承担此类大型测序项目。就目前正在发展的几种高通量测序技术进行了综合阐述,并比较了各种测序技术的优缺点,最后对其应用领域作了简单介绍。
The development of Sanger sequencing has brought a powerful tool for human being to decipher the genetic code. How- ever,the arrival of high-throughput sequencing over the past several years highly challenges the traditional method,not only in cost but also in efficiency. A race toward the $ 1 000 genome dramatically accelerates the speed of technological innovation. Therefore,next gen- eration sequencing will allow us to draw a complete genome map, compare gene expression under different conditions,and demonstrate sequence variation between or within species,all of which would open out a new avenue for biological or medical research. At the same time, with the highly-developed sequencing platforms, nearly every research team would have the opportunity to carry out such research. In this paper,we provide a snapshot of these new approaches, discuss the advantages and limitations of them, and also illustrate their applications in various fields.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第10期76-81,共6页
Biotechnology Bulletin
基金
国家自然科学基金项目(30730008
30970208
40976081)
"973"计划项目(2007CB815703)
中山大学重点培育计划项目
有害生物控制与资源利用国家重点实验室创新课题
教育部留学归国人员科研启动基金
中山大学重点培育计划
关键词
第二代测序
第三代测序
基因组
转录组
重测序
Second generation sequencing Third generation sequencing Genome Transcriptome Re-sequencing
