摘要
为明确栽培羌活最佳采收年限与采收时间,推进羌活产业化高质发展,该研究建立了栽培羌活药材特征图谱,并结合化学计量学和熵权-灰色关联度分析,对不同栽培年限与采收期的羌活农艺性状和药材质量指标进行了差异性分析。通过对照品比对共识别了10个共有峰,分别为绿原酸、4-香豆酸、阿魏酸、异紫花前胡苷、紫花前胡苷、异绿原酸B、羌活醇、阿魏酸苯乙醇酯、异欧前胡素和镰叶芹二醇,不同栽培年限羌活药材特征图谱与对照特征图谱的相似度均大于0.932。主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)显示,一至三年生栽培羌活离散度高质量不稳定,而四年生栽培羌活在不同采收期内质量较为稳定,表明药材中相关化合物已积累到平台期,这证实羌活药材最佳栽培年限为4年。熵权-灰色关联度分析表明,四年生栽培羌活不同采收时期下质量从高到低依次为11月、12月、10月、8月、7月、9月,表明11月为栽培羌活的最佳采收时间。该研究结果确定了栽培羌活药材的适宜种植年限与最佳采收时间,为栽培羌活生产指导及其质量标准完善提供了科学依据。
To determine the optimal cultivation duration and harvest period for cultivated Notopterygium incisum and promote its industrial development,this study established a characteristic chromatographic profile of cultivated N.incisum and employed chemometrics combined with entropy-weighted grey correlation analysis to assess differences in agronomic traits and quality indicators across different cultivation years and harvest periods.By comparing with reference substances,ten common peaks were identified,including chlorogenic acid,p-coumaric acid,ferulic acid,marmesinin,nodakenin,isochlorogenic acid B,notopterol,phenethyl ferulate,isoimperatorin,and falcarindiol.The similarity between the characteristic chromatographic profiles of N.incisum at different cultivation years and the reference profile was all above 0.932.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)revealed that the quality of 1-to 3-year-old cultivated N.incisum was highly dispersed and unstable,whereas the quality of 4-year-old cultivated N.incisum remained relatively stable across different harvest periods.This suggests that the accumulation of relevant compounds in the medicinal material had reached a plateau,confirming that the optimal cultivation period for N.incisum is four years.Entropy-weighted grey correlation analysis indicated that the quality of 4-year-old cultivated N.incisum across different harvest periods ranked from highest to lowest as follows:November,December,October,August,July,and September,demonstrating that November is the optimal harvest time.The findings of this study establish the suitable cultivation duration and optimal harvest period for N.incisum,providing a scientific basis for cultivation guidance and quality standardization.
作者
王京城
孙洪兵
刘腾
朱文涛
王红兰
周毅
王维彦
杨萍
蒋舜媛
WANG Jing-cheng;SUN Hong-bing;LIU Teng;ZHU Wen-tao;WANG Hong-lan;ZHOU Yi;WANG Wei-yan;YANG Ping;JIANG Shun-yuan(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China)
出处
《中国中药杂志》
北大核心
2025年第14期3878-3886,共9页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2023YFC3503803)
四川省中医药管理局科技项目(2023zd029)
阿坝州科技计划项目(R23TJNLJS0005)。
关键词
栽培羌活
特征图谱
质量评价
熵权-灰色关联度分析
采收期
cultivated Notopterygium incisum
specific chromatogram
quality evaluation
entropy weight-gray correlation analysis
harvesting period
