摘要
目的建立不同基原羌活标准汤剂UHPLC指纹图谱分析方法,为其质量评价提供依据。方法通过UHPLC构建26批羌活标准汤剂和宽叶羌活标准汤剂的指纹图谱,采用中药色谱指纹图谱相似度评价系统(2012A版)对指纹图谱进行相似度评价,并进行聚类分析、主成分分析及正交偏最小二乘-判别分析。结果选取了14个共有峰,指认其中11个成分;羌活、宽叶羌活标准汤剂的相似度分别为0.939~0.988、0.943~0.989;化学计量学结果显示,26批羌活标准汤剂样品分为2类,并筛选出紫花前胡苷、6′-O-(反式阿魏酰基)-紫花前胡苷、阿魏酸、异绿原酸B、绿原酸5个对组分差异影响较大的成分。结论建立的方法可快速区分和评价不同基原羌活标准汤剂和鉴别其相关伪品,为羌活相关中药制剂质量控制提供参考。
OBJECTIVE To establish a UHPLC fingerprint analysis method for standard decoction of Notopterygii Rhizoma et Radix(NRR)from different origins,so as to provide a theoretical basis for its quality evaluation.METHODS The fingerprints of 26 batches of Notopterygium incisum Ting ex H.T.Chang and Notopterygium franchetii H.de Boiss.standard decoction samples were established by UHPLC,and the similarity evaluation of the fingerprints was carried out using the traditional Chinese medicine chromatographic fingerprint similarity evaluation system(2012A version),and cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis were performed.RESULTS Fourteen chromatographic peaks were selected as common peaks,and 11 components were identified.The similarity of standard decoction samples of Notopterygium incisum Ting ex H.T.Chang and Notopterygium franchetii H.de Boiss.were 0.939−0.988 and 0.943−0.989,respectively.The results of chemometrics showed that the 26 batches of NRR standard decoction samples were clustered into 2 types and 5 components with large differences in composition were screened out including nodakenin,6′-O-trans-feruloylnodakenin,ferulic acid,isochlorogenic acid B and chlorogenic acid.CONCLUSION The established methods can quickly identify and evaluate the origins of standard decoction of NRR and counterfeits,it provides reference for the quality control of Chinese medicinal preparation related to NRR.
作者
吕渭升
位翠杰
卢晓莹
何广铭
罗睿
李振雨
贾小舟
陈向东
孙冬梅
LYU Weisheng;WEI Cuijie;LU Xiaoying;HE Guangming;LUO Rui;LI Zhenyu;JIA Xiaozhou;CHEN Xiangdong;SUN Dongmei(Guangdong Yifang Pharmaceutical Co.,Ltd.,/Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Foshan 528244,China)
出处
《中国现代应用药学》
北大核心
2025年第14期2417-2424,共8页
Chinese Journal of Modern Applied Pharmacy
基金
国家工信部产业技术基础公共服务平台项目——中药全产业链质量技术服务平台(2022-230-221)。
关键词
羌活
宽叶羌活
标准汤剂
指纹图谱
化学计量学
聚类分析
主成分分析
正交偏最小二乘-判别分析
Notopterygium incisum ting ex H.T.Chang
Notopterygium franchetii H.de Boiss.
standard decoction
fingerprint
chemometrics
cluster analysis
principal component analysis
orthogonal partial least squares-discriminant analysis
