摘要
为了研究不同配比羌活-独活药对与单味药中挥发油成分差异,采用盐辅助水蒸气蒸馏法提取挥发油,GC-MS分析羌活、独活单味药及羌活-独活药对不同配比挥发性成分,随后利用化学计量学评价挥发性物质成分差异,并进行抗氧化实验。结果表明,通过GC-MS共鉴定出79种挥发性物质,当羌活-独活药对配比为2:1时,得到50种挥发性物质及7种新增化合物。各组挥发性成分差异贡献较大的化合物为间甲酚、(1R)-(+)-α-蒎烯、(R)-(+)-柠檬烯、β-蒎烯和(1S)-(-)-β-蒎烯。羌活、独活及3种不同配比药对具有较好的清除自由基能力。该研究确认了2:1的羌活-独活药对鉴定挥发性物质最多以及导致不同药对配比差异的4种关键化合物,可为羌活-独活药对科学配伍提供参考。
To investigate the changes in volatile oil composition between different ratios of Notopterygium incisum-Angelicae pubescentis drug pairs and single drug,salt-assisted water vapor distillation was employed to extract the volatile oils and the volatile components were analyzed using GC-MS.Differences in volatile substance compositions were then evaluated using stoichiometry,and antioxidant experiments were performed.The findings demonstrated that 79 volatile substances in total were detected by GC-MS,and50 volatile substances and 7 novel compounds found when the Notopterygium incisum-Angelicae pubescentis drug pair ratio was 2:1.Compounds such as m-cresol,(1R)-(+)-α-pinene,(R)-(+)-limonene,β-pinene,and(1S)-(-)-β-pinene had a significant role in determining volatile component variations between groups.Notopterygium incisum,Angelicae pubescentis,and three different dosing pairs exhibited superior free radical scavenging activity.In conclusion,the study found the most volatile substances in the 2:1 Notopterygium incisum-Angelicae pubescentis drug pairs,as well as the four essential compounds that caused changes in the ratios of the different pairings,which may be used as a reference for scientific compounding of Notopterygium incisum-Angelicae pubescentis drug pairs.
作者
张育珍
张维迅
吴曼
张博
唐一梅
贺茂芳
张剑
ZHANG Yu-zhen;ZHANG Wei-xun;WU Man;ZHANG Bo;TANG Yi-mei;HE Mao-fang;ZHANG Jian(School of Pharmacy,Xi'an Medical university,Xi'an 710021,China;International College of Pharmaceutical Innovation,Soochow University,suzhou 215222,China)
出处
《化学研究与应用》
北大核心
2025年第3期735-742,共8页
Chemical Research and Application
基金
陕西省大学生创新训练计划项目(S202411840058)资助。
关键词
羌活-独活药对
挥发油
抗氧化
Notopterygium incisum-Angelicae pubescentis drug pair
volatile oil
antioxidant
