摘要
建立同时测定发酵液中终产物白藜芦醇和中间产物4-香豆酸含量的方法。利用高效液相色谱仪进行含量测定,色谱柱为Venusil XBP C_(18) (2),流动相为1‰三氟乙酸乙腈溶液和1‰三氟乙酸水溶液,梯度洗脱,检测波长306 nm。方法线性良好(R^(2)>0.999),精密度试验相对标准偏差小于3.0%,平均回收率大于97%,白藜芦醇和4-香豆酸的最低检出限分别为0.03 mg·mL^(-1)和0.02 mg·mL^(-1)。本方法快速简单,重复性好,为合成生物行业测定发酵液中白藜芦醇和4-香豆酸的浓度提供检测依据。
A method was established for the simultaneous determination of resveratrol and 4-coumaric acid,the final product,in the fermentation broth.The content was determined by high-performance liquid chromatography with Venusil XBP C_(18)(2)on the column,1‰acetonitrile trifluoroacetic acid and 1‰ trifluoroacetic acid in aqueous 1‰,gradient elution,and detection wavelength of 3062 nm.The linearity of the method was good(R>0.999),the relative standard deviation of the precision test was less than 3.0%,the average recovery rate was more than 97%,and the minimum detection limits of resveratrol and 4-coumaric acid were 0.03 mg·mL^(-1) and 0.02 mg·mL^(-1),respectively.This method is fast,simple,and reproducible,which provides a basis for the determination of the concentration of resveratrol and 4-coumaric acid in fermentation broth in the synthetic biology industry.
作者
欧海军
彭勇
蔡耀旻
杨艳
OU Haijun;PENG Yong;CAI Yaoming;YANG Yan(Ningbo J&S Botanics Inc,Ningbo 315804,China)
出处
《现代食品》
2025年第15期183-187,共5页
Modern Food
基金
2022年度北仑区(开发区)人才项目“‘揭榜挂帅’:虎杖活性提取物工程菌株的构建及其生产工艺技术研究”。
关键词
白藜芦醇
4-香豆酸
高效液相色谱法
resveratrol
4-coumaric acid
high-performance liquid chromatography
