摘要
【目的】探明5个抗稻瘟病基因(Pi2、Pib、Pita、Pigm、Pi9)在86份籼稻资源中的分布及不同基因型组合的抗性效应,为抗稻瘟病品种的选育及稻瘟病抗性基因的有效聚合提供理论依据。【方法】利用5对扩增条带清晰的功能标记,对86份黄淮稻区籼稻的稻瘟病抗性基因进行检测,并结合人工接种鉴定,明确5个抗性基因的分布和该地区中、高抗性的基因型组合。【结果】5个抗性基因Pi2、Pib、Pita、Pigm、Pi9在86份籼稻中的分布频率分别为98.80%、46.50%、55.80%、48.80%、1.20%。在86份供试材料中共检测出10种基因组合,分别为不含任何抗性基因、Pi2、Pi2+Pita、Pi2+Pib、Pi2+Pigm、Pi2+Pib+Pita、Pi2+Pi9+Pigm、Pi2+Pib+Pigm、Pi2+Pita+Pigm、Pi2+Pib+Pita+Pigm。接种鉴定为中抗以上的材料共有33份,占比38.37%;含有3种基因型Pi2+Pib+Pita、Pi2+Pita+Pigm、Pi2+Pib+Pita+Pigm的材料中,中抗以上材料占比分别为50%、80%和100%。【结论】Pi2在86份籼稻中的分布频率最高,为98.80%;抗性较好的3个基因型组合分别是Pi2+Pib+Pita、Pi2+Pita+Pigm和Pi2+Pib+Pita+Pigm。
[Objective]To investigate the distribution of five rice blast resistance genes(Pi2,Pib,Pita,Pigm,Pi9)in 86 indica rice accessions and evaluate the resistance effects of different genotypic combinations,providing a theoretical basis for breeding rice blast-resistant varieties and effective pyramiding of resistance genes.[Methods]Using five pairs of functional markers with clear amplification bands,blast resistance genes in 86 indica rice accessions from the Huang-Huai rice region were detected,and combined with artificial inoculation assays,the distribution of five resistance genes and the genotypic combinations conferring moderate to high resistance in this region were identified.[Results]The distribution frequencies of the five resistance genes Pi2,Pib,Pita,Pigm and Pi9 in the 86 indica rice accessions were 98.80%,46.50%,55.80%,48.80%,and 1.20%,respectively.Ten genotypic combinations were identified among the 86 tested materials,no resistance genes,Pi2,Pi2+Pita,Pi2+Pib,Pi2+Pigm,Pi2+Pib+Pita,Pi2+Pi9+Pigm,Pi2+Pib+Pigm,Pi2+Pita+Pigm and Pi2+Pib+Pita+Pigm,respectively.Inoculation assays showed that 33 accessions exhibited moderate or higher resistance,accounting for 38.37%;among the materials with three genotypic combinations Pi2+Pib+Pita,Pi2+Pita+Pigm and Pi2+Pib+Pita+Pigm,the proportions of accessions with moderate or higher resistance were 50%,80%and 100%,respectively.[Conclusion]The Pi2 gene exhibits the highest distribution frequency(98.80%)among the 86 indica rice accessions;the three genotypic combinations confering superior resistance are Pi2+Pib+Pita,Pi2+Pita+Pigm and Pi2+Pib+Pita+Pigm.
作者
陈春
李凡
郭新亚
王飞飞
王磊
陈卫军
CHEN Chun;LI Fan;GUO Xinya;WANG Feifei;WANG Lei;CHEN Weijun(Suqian Institute of Agricultural Sciences,Jiangsu Academy of Agricultural Sciences,Suqian 223800,China)
出处
《北方农业学报》
2025年第2期1-9,共9页
Journal of Northern Agriculture
基金
宿迁市自主创新项目(SQCX202104)
宿迁市科技计划项目(L202202)。
关键词
籼稻
稻瘟病
抗性基因
抗性分析
基因型
Indica rice
Rice blast
Resistance genes
Resistance analysis
Genotype
